Introduction/Background Transcription factors encoded by the OCT4 and SOX2 genes are involved in the regulation of human embryonic stem cell function and may play an important role in tumor progression. Mutant versions of the Myc gene are found in many tumors. Our purpose was to study relative expression of genetic loci encoding transcription factors in intact and pathological tissue of the uterine body.
Methodology Biopsy tissues of the uterus (tumor - TT and intact - IT) were studied in 27 patients aged 38–72 years with histologically verified endometrial cancer (EC). Relative expression of 3 genetic loci encoding OCT4, SOX2 and c-Myc transcription factors was determined by RT-qPCR. The primer design for RT-qPCR was performed using NCBI GenBank DNA reference sequences. ACTB was used as the reference gene.
Results Patients with G1 EC, 45-68 years, showed an increased SOX2 expression (by 178%, p<0.05); G2 EC, 38–69 years - no significant changes in expression of the genetic loci; G3 EC, 55–63 years - expression of SOX2 and c-Myc increased by 63% and 130% (p<0.05) respectively in TT comparative to IT. Expression of gene pairs OCT4 and SOX2, OCT4 and c-Myc, c-Myc and SOX2 in both IT and TT of different grades demonstrated a strong positive correlation from +0.940 to +0. 998. OCT4, SOX2, and c-Myc expression was 35, 7, and 2 times higher (p<0.005), respectively, in G3 TT compared to G1 TT; OCT4, SOX2, and c-Myc expression was 32, 12 and 2 times higher in G3 IT compared to G1 IT.
Conclusion The maximal increase was observed in the OCT4 gene expression, confirming its status as a marker of undifferentiated cells. In tumors of higher grades, changes in OCT4, SOX2, and c-Myc expression affected uterine IT, however with less intensity, especially in later stages of the disease.
Disclosure Nothing to disclose.
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