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EP578 Detection of lymph vessel invasion and blood vessel invasion using double D2–40 and CD31 immunohistochemistry reveals independent prognostic significance of blood vessel invasion in endometrial cancer
  1. A Mihaljević Ferari1,
  2. M Klarić2,
  3. M Krašević3,
  4. S Eminović3,
  5. J Marušić1,
  6. I Plišić2,
  7. G Sopta2 and
  8. E Babarović3
  1. 1Clinical Department of Radiotherapy and Oncology
  2. 2Clinical Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka
  3. 3Department Pathology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia


Introduction/Background Lymph-vascular space invasion (LVSI) is adverse prognostic factor in endometrial cancer (EC). Due to lack in histologic criteria and interobserver variability its role in administering adjuvant treatment and predicting recurrence isn´t well defined. The aim of our study was to utilize sensitive immunohistochemical method for separate detection of lymph vessel invasion (LVI) and blood vessel invasion (BVI) in patients with EC and to investigate their prognostic impact.

Methodology We retrospectively identified from the institutional database 217 patients with EC and with complete follow-up, between 2002 and 2006. Tumor specimens were retrieved and two-colored double staining immunohistochemistry (D2-40/CD31) was performed to highlight lymphatic and blood vessels in the same slide. Tumors were divided in three groups: LVSI negative, LVI positive and BVI positive.

Results In the original pathology reports, LVSI was misclassified in 36 (16.6%) tumors. In this study by using immunohistochemistry LVI was detected in 64 (29.5%) and BVI in 32 (14.7%) cases. All tumors with positive BVI also had accompanying LVI. BVI had the strongest impact on the risk for recurrence (OR 9.27 CI 4.47–19.22; p<0.0001) and was, also significantly associated with decreased survival outcomes (overall survival, 59% compared with 96% in LVI group and 98% in LVSI negative group, p<0.0001 and disease-free survival, 5-year rate 42% compared with 96% in both other groups, p<0.0001) in univariate analysis. In multivariate analysis (including: age, tumor type, grade, stage, treatment) BVI remained independent prognostic factor for recurrence (HR 7.1 CI 2.9–16.8), overall survival (HR 8.2 CI 4.1–16.4) and disease-free survival (HR 6.2 CI 2.9–12.8).

Conclusion Our results suggest that BVI could have greater clinical value in contrast to isolated LVI, and that therapeutic decisions should be based on its presence. We hope that these preliminary results will encourage further application of this inexpensive, readily available technique.

Disclosure Nothing to disclose.

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