Introduction/Background In the presence of high risk HPV, E7 oncogene causes the overexpression of p16 and Ki-67 is an indicator for cell proliferation. This study aims to evaluate HPV positive women in aspect of p16 and Ki-67 presence in tissues.
Methodology This is a retrospective review of 287 patients who tested positive for HPV DNA.
Results Colposcopic biopsies revealed normal histopathological findings in 28 patients (9.8%), cervicitis in 48 patients (16.7%), cervical intraepithelial lesion (CIN) 1 in 178 patients (62.0%), CIN2 in 26 patients (9.1%), CIN3 in four patients (1.4%) and carsinoma in situ (CIS) in three patients (1.0%). Cervical tissue positivity for p16 was 2.6% in patients with benign histopathological findings, 16.3% in patients with CIN1, 92.3% in patients with CIN2, 100% in patients with CIN3 and 100% in patients with CIS. Cervical tissue positivity for Ki-67 was 42.1% in patients with benign histopathological findings, 77.5% in patients with CIN1, 96.1% in patients with CIN2, 100% in patients with CIN3 and 100% in patients with CIS. The sensitivity and specificity of p16 positivity for CIN1, CIN2, CIN3 and CIS were 25.1% (95% CI: 19.4%–31.5%) and 97.4% (95% CI: 90.8%–99.7%) respectively. When p16 positivity was combined with Ki-67 positivity, the sensitivity decreased to 20.4% (95% CI: 15.2%–26.5%) and the specificity was 97.4% (95% CI: 90.8%–99.7%). The sensitivity and specificity of p16 positivity for CIN2, CIN3 and CIS were 93.9% (95% CI: 79.8%–99.3%) and 90.6% (95% CI: 86.3%–93.9%) respectively. When p16 positivity was combined with Ki-67 positivity, the sensitivity decreased to 90.9% (95% CI: 75.7%–98.1%) and the specificity increased to 94.1% (95% CI: 90.5%–96.7%).
Conclusion The presence of p16 and Ki-67 in cervical tissues has a significant role in the detection of precancerous lesions and may help to predict the progression of precancerous lesions into malignancy.
Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.