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EP334 SENTIX – sentinel lymph nodes in patients with cervical cancer: SLN detection and the false negative rate of SLN frozen section (CEEGOG-CX01; ENGOT-CX2; NCT02494063)
  1. R Kocian1,
  2. C Köhler2,
  3. J Klat3,
  4. A Germanova1,
  5. A Plaikner2,
  6. S Bajsova3,
  7. S Marnitz2,
  8. M Ostojich4,
  9. I Zapardiel5,
  10. B Gil-Ibañez6,
  11. B Sehnal7,
  12. A Petiz8,
  13. R Pilka9,
  14. A Martin-Martinez10,
  15. J Presl11,
  16. A Buda12,
  17. L van Lonkhuijzen13,
  18. L Minar14,
  19. M Barahona15,
  20. D Wydra16,
  21. P Blecharz17,
  22. P Dundr18,
  23. L Dusek19 and
  24. D Cibula1
  1. 1Gynecologic Oncology Center, Department of Obstetrics and Gynecology, First Faculty of Medicine, Charles University and General University Hospital in Prague, CEEGOG*, Prague, Czech Republic
  2. 2Department of Special Operative and Oncologic Gynaecology, Asklepios-Clinic Hamburg, Hamburg, Germany
  3. 3Department of Obstetrics and Gynecology, University Hospital Ostrava, CEEGOG*, Ostrava, Czech Republic
  4. 4Department of Gynecology, Institute of Oncology Angel H Roffo University of Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
  5. 5Gynecologic Oncology Unit, La Paz University Hospital, Madrid
  6. 6Unit of Gynecological Oncology, Institute Clinic of Gynecology, Obstetrics and Neonatology (ICGON), Hospital Clinic of Barcelona, Barcelona, Spain
  7. 7Department of Obstetrics and Gynecology, Bulovka Hospital, First Faculty of Medicine, Charles University, CEEGOG*, Prague, Czech Republic
  8. 8Department of Gynecology, Francisco Gentil Portuguese Oncology Institute, Porto, Portugal
  9. 9Department of Obstetrics and Gynecology, University Hospital Olomouc, Olomouc, Czech Republic, CEEGOG*, Olomouc, Czech Republic
  10. 10Hospital Universitario Materno Infantil de Canarias, Las Palmas de Gran Canaria, Spain
  11. 11Department of Gynaecology and Obstetrics, University Hospital Pilsen, Charles University, CEEGOG*, Pilsen, Czech Republic
  12. 12Clinic of Obstetrics and Gynecology, San Gerardo Hospital, Monza, Italy
  13. 13Center for Gynecologic Oncology, Academic Medical Centre, Amsterdam, The Netherlands
  14. 14Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, Brno, Czech Republic
  15. 15Department of Gynecology, University Hospital of Bellvitge, Biomedical Research Institute of Bellvitge, University of Barcelona, Barcelona, Spain
  16. 16Department of Gynecology, Gynecologic Oncology and Gynecologic Endocrinology, Medical University of Gdansk, Gdansk
  17. 17Department of Gynecologic Oncology, Centre of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Department, Cracow, Poland
  18. 18Institute of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague
  19. 19Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic


Introduction/Background SENTIX is a prospective cohort multicenter international study on sentinel lymph node (SLN) biopsy without pelvic lymph node dissection (PLND) in patients with early-stage cervical cancer. SLN frozen section (FS) and pathological ultrastaging were mandatory. Samples from SLN were reviewed centrally for pathological assessment quality control. Only sites experienced in SLN biopsy technique were eligible.

Methodology In total, 47 sites from 18 countries participated in the study. Patients with stages T1a1/LVSI+ - T1b2 (<4 cm or ≤2 cm for fertility sparing), with common tumor types and no suspicious lymph nodes on imaging were pre-registered in the study. Patients remained registered after the surgery if SLN were detected on both sides and if SLN were negative on frozen section. Blue dye, radioactive tracer, indocyanine -green (ICG) or their combinations were all eligible tracers for SLN detection. SLN ultrastaging protocol included a complete processing of all SLN tissue in slices of 2 mm thickness, 2 sections in 150 µm from each block until no tissue left, one stained with H&E and second examined immunohistochemically.

Results Data from 372 patients were analyzed who were pre-registered at the time when the number of cases treated per protocol reached 300. Patient characteristics are shown in table 1. Bilateral detection rate reached 91%. table 2 shows factors influencing SLN detection. Frozen section detected macrometastases (MAC) in 22 cases. SLN ultrastaging found additional 7 cases with MAC, 19 with micrometastases (MIC) and 11 with isolated tumor cells (ITC). Sensitivity of FS was 75.9% for the detection of MAC and 37.3% for any type of SLN involvement.

Conclusion High bilateral detection can be achieved in sites experienced in the SLN biopsy technique. We did not confirm a higher detection rate using ICG. FS failed to detect 26/48 (54%) of cases with MIC or MAC in SLN.

Disclosure This work was supported by a grant from the Czech Research Council (No 16-31643A). Conflict of interest: None of the authors declare a conflict of interest.

Abstract EP334 Table 1

Preoperative characteristics of patients (N=372)

Abstract EP334 Table 2

Factors influencing bilateral detection rate of SLN

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