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226 Telomerase endometrial cells activity in hyperplastic processes
  1. Z Chumak1,2,
  2. A Zelinsky2,
  3. V Artyomenko2 and
  4. N Shapoval2
  1. 1Odessa City Climacteric Centre, Odessa, Ukraine
  2. 2Odessa National Medical University, Obstetrics and Gynecology, Odessa, Ukraine


Objectives Endometrial hyperplastic processes (EHP) like a combination of stromal and glandular tissue components changes has properties both for self-healing, recurrence and for malignancy, depending on morphological changes degree with markers and predictors conducted research at cellular, molecular-genetic, immune-hystochemical levels.

Telomerase activity estimation in endometrial cells at EHP.

Methods According to WHO classification (1994) following groups were formed: 1 - simple hyperplasia -7 samples; II - complex hyperplasia -8; III - simple atypical hyperplasia -8; IV - complex atypical hyperplasia -7 specimens. The control groups with morphologically unchanged endometrium -11 samples: V group - proliferation phase - 6 specimens; VI group - secretion phase-5 samples. Relative telomerase activity was determined using real-time PCR with SYBR Green dye (RQ-TRAP) according to Wege et al. methodic. To improve the study accuracy we used from 4 to 6 cells samples and calculated their average value in each experiment.

Results Women age was 45.38±1.66 years. In simple hyperplasia endometrial specimens, telomerase activity was 1.22±0.10a.u. (pI-IV<0,05), complex hyperplasia - 1.35±0.07a.u. (pII-pIV<0,05), simple atypical hyperplasia - 1.23±0.08 a.u. (pIII-pIV<0,05). The complex atypical hyperplasia telomerase activity increase reaching 1.54±0.05a.u. (pIV-pV<0,05; pIV-pVI<0,05) was statistically significant. Therefore, telomerase reactivation in atypical complex hyperplasia can confirm the proliferative stage telomeres lengthening, increasing the ability for cell division, on the other hand, telomerase ability to control cell division in endometrial tissue.

Conclusions The parallelism presence between telomerase activity in approximately 85% of human tumors and telomerase reactivation in endometrial cells with complex atypical hyperplasia makes it possible to determine its activity fact like early malignancy marker.

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