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Effects of a Small Interfering RNA Targeting YKL-40 Gene on the Proliferation and Invasion of Endometrial Cancer HEC-1A Cells
  1. Li-li Li, MM*,
  2. Jiang-tao Fan, MD*,
  3. Da-hai Li, MM* and
  4. Yan Liu, MM
  1. *Department of Gynecology, First Affiliated Hospital of Guangxi Medical University, Nanning; and
  2. Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  1. Address correspondence and reprint requests to Jiang-tao Fan, MD, Department of Gynecology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. E-mail: jiangtao_fan1969@163.com.

Abstract

Objective The purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells.

Methods We used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40. MTT, migration, and invasion assays were performed to identify the effects of si-YKL-40 on the proliferation, migration, and invasive abilities of the HEC-1A cells.

Results mRNA expression of YKL-40 was down-regulated in HEC-1A cells after transfection with si-YKL-40 (P < 0.05). The proliferation, migration, and invasive abilities of HEC-1A cells were inhibited by siRNA (P < 0.05).

Conclusions YKL-40 targeting siRNA specifically blocks the activity of YKL-40 in human EC HEC-1A cells, resulting in tumor suppression. This indicates that YKL-40 might serve as a potential small molecule target in the treatment of EC.

  • YKL-40
  • siRNA
  • Endometrial cancer
  • Proliferation
  • Migration
  • Invasion

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Footnotes

  • This work was supported by the Natural Scientific Foundation of China: The experimental study on the regulatory function of YKL-40 in the endometrial carcinoma angiogenesis and antiapoptosis (No. 81360388).

  • The authors declare no conflicts of interest.

  • Jiang-tao Fan conceived the study and analyzed data. Li-li Li, Da-hai Li, and Yan Liu carried out the experiments and analyzed data. Li-li Li wrote the manuscript. All authors read and approved final manuscript.

  • Ethics approval and consent to participate: The Guangxi Medical University Ethics Committee approved the above research and the subjects provided informed consent.

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