Objective The purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells.
Methods We used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40. MTT, migration, and invasion assays were performed to identify the effects of si-YKL-40 on the proliferation, migration, and invasive abilities of the HEC-1A cells.
Results mRNA expression of YKL-40 was down-regulated in HEC-1A cells after transfection with si-YKL-40 (P < 0.05). The proliferation, migration, and invasive abilities of HEC-1A cells were inhibited by siRNA (P < 0.05).
Conclusions YKL-40 targeting siRNA specifically blocks the activity of YKL-40 in human EC HEC-1A cells, resulting in tumor suppression. This indicates that YKL-40 might serve as a potential small molecule target in the treatment of EC.
- Endometrial cancer
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This work was supported by the Natural Scientific Foundation of China: The experimental study on the regulatory function of YKL-40 in the endometrial carcinoma angiogenesis and antiapoptosis (No. 81360388).
The authors declare no conflicts of interest.
Jiang-tao Fan conceived the study and analyzed data. Li-li Li, Da-hai Li, and Yan Liu carried out the experiments and analyzed data. Li-li Li wrote the manuscript. All authors read and approved final manuscript.
Ethics approval and consent to participate: The Guangxi Medical University Ethics Committee approved the above research and the subjects provided informed consent.