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miR-375 Affects the Proliferation, Invasion, and Apoptosis of HPV16-Positive Human Cervical Cancer Cells by Targeting IGF-1R
  1. Xiao Yu, MS,
  2. Weihong Zhao, MS,
  3. Xin Yang, PhD,
  4. Zhilian Wang, MS and
  5. Min Hao, MD
  1. Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, Taiyuan, China.
  1. Address correspondence and reprint requests to Min Hao, MD, Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, No 382 Wuyi Rd, Taiyuan 030001, China. E-mail: 2yuanhaomin{at}163.com.

Abstract

Objective The aim of this study was to examine the relationship between miR-375 expression and the proliferation, apoptosis, and migration of cervical cancer cells. To further explore the potential target gene of miR-375, insulin-like growth factor 1 receptor (IGF-1R) was detected in miR-375 overexpressed and inhibited cervical cancer cells, which clarified the potential mechanism of miR-375 in the growth and development of cervical cancer.

Methods In a cervical cancer cell line (Caski), miR-375 overexpression and knockdown were achieved by transfection with a synthetic miR-375 mimic or miR-375–targeting inhibitor oligonucleotides, respectively, using siRNA-Mate transfection reagents. Real-time Polymerase Chain Reaction was performed to detect the expression level of miR-375. The functional effects of miR-375 on cell proliferation, migration, and apoptosis were evaluated using a Cell Counting Kit (CCK-8) and through scratch wound tests and apoptosis assays, respectively. Western blotting was performed to detect the expression level of the IGF-1R protein.

Result Transfection with the miR-375 mimic significantly upregulated the expression of miR-375 by approximately 7.76-fold (P < 0.05), reduced cell proliferation and migration (P < 0.05), increased apoptosis (P < 0.05), and decreased the expression of the IGF-1R protein by 24.73% (P < 0.05) compared with the negative control. In contrast, transfection of the miR-375 inhibitor decreased the expression of miR-375 by 14.39% (P < 0.05), significantly increased cell proliferation and migration (P < 0.05), significantly reduced the cell apoptosis (P < 0.05), and upregulated the expression of the IGF-1R protein by 2.29-fold (P < 0.05). The cells transfected with the negative control showed no significant changes compared with the blank control for each parameter (P > 0.05).

Conclusions miR-375 plays an important role in the tumorigenesis and development of cervical cancer. IGF-1R might represent a target gene of miR-375 in cervical cancer.

  • miR-375
  • Cervical cancer
  • IGF-1R
  • HPV16 positive

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Footnotes

  • The authors declare no conflicts of interest.

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