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Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4+CD25+FoxP3+Regulatory T Cells In Vivo
  1. Xiaoyun Chen, MD*,
  2. Bingdong Zhu, PhD,
  3. Yanping Luo, PhD,
  4. Dongdong Zhang, MD*,
  5. Lang Zhang, MD§,
  6. Huihui Zhu, MD,
  7. Chunyan Hao, PhD,
  8. Yuzhen Guo, PhD* and
  9. Huiling Liu, PhD
  1. *Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu Province, China; Institutes of
  2. Pathogen Biology and
  3. Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu Province, China;
  4. §Institute of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu Province, China; and
  5. Department of Obstetrics and Gynecology, Gansu Provincial Hospital, Lanzhou, Gansu Province, China.
  1. Address correspondence and reprint requests to Yuzhen Guo, PhD, Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu Province, China. E-mail: guoyz@lzu.edu.cn; Huiling Liu, PhD, Department of Obstetrics and Gynecology, Gansu Provincial Hospital, Lanzhou, Gansu Province, China. E-mail: liuhuiling75@163.com.

Abstract

Aim To investigate the immunotherapeutic effectiveness of adenoviral vector expressing mouse interleukin (IL)-28B (Ad-mIL-28B) against cervical cancer and its mechanism.

Method U14 cervical cancer cell–bearing mice were treated with Ad-mIL-28B. Meanwhile, whole cell vaccine was prepared by repeated freezing and thawing U14 cells. Then CD4+CD25+FoxP3+regulatory T (Treg) cells were evaluated by flow cytometry. Tumor volume and metastasis in BALB/c and C57BL/6j mice were detected.

Results Ad-mIL-28B treatment significantly decreased the number of CD4+CD25+FoxP3+Treg cells. Subsequently, there was a significant decrease in the size of tumor tissue and the numbers of heteromorphic tumor cells. The tumor metastasis in the lung and liver of the Ad-mIL-28B group also decreased. However, there was no therapeutic effect observed for whole cell vaccine on U14 tumor–bearing mice.

Conclusion Interleukin-28B can inhibit the growth and metastasis of cervical cancer in U14 tumor–bearing mice by down-regulating Treg cells.

  • IL-28B
  • Cervical cancer
  • Regulatory T cells
  • Immunotherapy
  • U14 cell

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