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Dehydrocostus Lactone Inhibits Proliferation, Antiapoptosis, and Invasion of Cervical Cancer Cells Through PI3K/Akt Signaling Pathway
  1. Enping Jiang, MD,
  2. Xiwen Sun, MD,
  3. Haixian Kang, MM,
  4. Liping Sun, MB,
  5. Weifang An, MB,
  6. Yunhong Yao, MM and
  7. Xinrong Hu, MD, PhD
  1. Department of Pathology, Cancer Research Institute of Guangdong Medical University, Dongguan, People’s Republic of China.
  1. Address correspondence and reprint requests to Xinrong Hu, MD, PhD, Cancer Research Institute of Guangdong Medical University, No. 1 Xincheng Rd, Dongguan, Guangdong Province 523808, People’s Republic of China. E-mail: huxinrong{at}163.com.

Abstract

Objectives Recent studies found that dehydrocostus lactone (DHC), a traditional Chinese medicine in curing chronic ulcer and inflammation, can inhibit several type of tumor cells. The purpose of this study was to define the role of DHC on cervical cancer cells and to explore its mechanism of action.

Methods We used DHC alone or in combination with PI3K/Akt-specific inhibitor LY294002 (LY) to treat Hela cells [human papillomavirus (HPV)-18 positive] and C33a cells (HPV negative). The proliferation, apoptosis, and Akt activation were assessed. Cell invasive ability was assayed in transwell chambers.

Results We found that DHC significantly inhibited proliferation, antiapoptosis, and invasion of both cells, and reduced the level of p-Akt phosphorylation in these cells, in a dose- or time-dependent manner. In addition, these inhibitions of DHC were significantly strengthened by LY.

Conclusions The result suggested that DHC plays a potent role in anticervical cancer in multiple biological aspects through PI3K/Akt signaling pathway, independently of HPV infection. This finding surely adds new knowledge to understand the role of DHC in fighting cancers.

  • Dehydrocostus lactone
  • Apoptosis
  • Akt
  • Cervical cancer

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Footnotes

  • Supported by the Dr Startup project of Guangdong Medical University (No. B2012063), by postdoctoral science foundation of China (2012M521582), and by the Science & Technology Innovation Project of Department of Education of Guangdong province (2012KJCX0057).

  • The authors declare no conflict of interest.

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