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Analyzing Simultaneous Positive Expression of EZH2 and P53 Protein to Improve Predictive Value in Cervical Squamous Cell Carcinoma
  1. Shu-Qin Chen, PhD, MD*,
  2. Hui-Min Zhang, MD,
  3. Jin-Bo Li, MD*,
  4. Hong-Ye Jiang, MD*,
  5. Li Fan, MD*,
  6. Ling-Zhi Kong, MD* and
  7. Shu-Zhong Yao, PhD, MD*
  1. *Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; and
  2. Department of Gynecology and Obstetrics, The People’s Hospital of Guizhou Province, Guizhou, China.
  1. Address correspondence and reprint requests to Shu-Zhong Yao, PhD, MD, Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, No 58, Zhongshan 2th Road, Guangzhou, China, 510080. E-mail:


Objective The current study was undertaken to investigate the predictive value of simultaneous enhancer of zeste homolog 2 (EZH2) and P53 expression in lesions of patients with cervical squamous cell carcinoma.

Methods Quantum dot double fluorescence staining was applied to detect EZH2 and P53 protein in biopsy tissue of 168 patients with cervical squamous cell carcinoma. The expression was classified into double positive (EZH2 and P53 were positively expressed), single positive (either EZH2 or P53 was positively expressed), and double negative (neither was positively expressed). The EZH2 and P53 expression, clinical stages of cervical cancer, lymph node metastasis, progression-free survival, and overall survival were analyzed.

Results A relationship of EZH2 and P53 expression with the clinical stage of cervical cancer and lymph node metastasis was indicated. Simultaneous detection of both proteins could partly predict prognosis, consistent with previous studies’ results. The current study demonstrated that the expression levels of EZH2 and P53 in tumor tissue and the proportion of cases with double-positive expression significantly increased with increasing clinical stages of cancer, also confirming up-regulated expression of EZH2 and P53 with increasing stages. These findings may suggest that the 2 proteins were involved in the development of cervical cancer, but the nature of their interaction is undefined. The increase of EZH2 and P53 expression in patients with lymph node metastasis indicated that they may be involved in metastasis of cervical cancer.

Conclusion Simultaneous positive EZH2 and P53 expression could improve the predictive value of a poor prognosis in cervical cancer.

  • EZH2
  • P53
  • Cervical cancer
  • Double quantum dot fluorescence staining
  • Prognosis

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  • Dr Shu-Qin Chen and Dr Hui-Min Zhang equally contributed to this work.

  • Supported by Science and Technology Planning Project of Guangdong Province, China (no. 2012B031800068) and Sun Yat-sen University Clinical Medical Research Project 5010, China (no. 2007010).

  • The authors declare no conflicts of interest.