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Beclin 1 Influences Cisplatin-Induced Apoptosis in Cervical Cancer CaSki Cells by Mitochondrial Dependent Pathway
  1. Yang Sun, MD*,
  2. Jia-hua Liu, BS*,
  3. Long Jin, M Med,
  4. Ling Pan, BS,
  5. Yu-xia Sui, M Med§,
  6. Yin Yang, BS* and
  7. Hong Shi, M Med*
  1. *Departments of Obstetrics and Gynecology, and
  2. Pathology, Fujian Provincial Hospital, Fujian Provincial Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China;
  3. Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Hospital, Fuzhou, Fujian, China; and
  4. §Department of Pharmacy, Fujian Provincial Hospital, Fujian Provincial Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.
  1. Address correspondence and reprint requests to Yang Sun, MD, Department of Obstetrics and Gynecology, Fujian Provincial Hospital, No. 134 Dong St, Fuzhou, Fujian 350001, PR China. E-mail: garyhanyu{at}sina.com.

Abstract

Purpose To investigate the role of Beclin 1 expression on the cisplatin-induced apoptosis in cervical cancer CaSki cells and to explore the potential mechanism underlying this effect.

Materials and Methods After overexpression or partial silencing of Beclin 1 in cervical cancer CaSki cells, the transfected group and the control group were treated with cisplatin for 24 hours. The percentage of apoptotic cells were assessed by flow cytometry. The mitochondrial membrane potential and activities of caspase-8/9/3 were detected by JC-1 fluorescence staining and colorimetry. The expression of cytochrome c was measured using a Western blot. The messenger RNA expression of Bax and Bcl-2 were detected by real-time quantitative reverse transcription polymerase chain reaction.

Results Expression of Beclin 1 protein was up-regulated in overexpressed transfectants of CaSki cells. After treatment with cisplatin, the Beclin 1 overexpression group led to the decrease of mitochondrial membrane potential and increase of activities of caspase-9 and caspase-3, and showed a greater increase in apoptosis than did the nontransfected group. Furthermore, Beclin 1 overexpression resulted in increased cytoplasmic cytochrome c and Bax expression and decreased mitochondrial cytochrome c and Bcl-2 expression.

Conclusion Overexpression of Beclin 1 in CaSki cells may influence cisplatin-induced apoptosis by mitochondrial dependent pathway.

  • Autophagy
  • Beclin 1
  • Apoptosis
  • Mitochondria

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Footnotes

  • This project was supported by the Natural Science Foundation of Fujian Province of China (No. 2011J01132).

  • The authors declare no conflicts of interest.

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