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Expression and Clinical Significance of Sperm-Associated Antigen 9 in Patients With Endometrial Carcinoma
  1. Peng Yu, MMedSc*,
  2. Lei Yan, MD*,
  3. Hui Zhang, MD*,
  4. Xiaoyan Lin, MD and
  5. Xingbo Zhao, MD*
  1. * Departments of Obstetrics and Gynecology and
  2. Pathology, Provincial Hospital Affiliated to Shandong University, Shandong, People’s Republic of China.
  1. Address correspondence and reprint requests to Xingbo Zhao, MD, Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Rd, Jinan, Shandong 250021, People’s Republic of China. E-mail: zhaoxingboandu{at}


Objective To investigate the expression and humoral immune response of sperm-associated antigen 9 (SPAG9) in endometri al carcinoma.

Methods Sperm-associated antigen 9 gene expression levels were evaluated in endometrial carcinoma, endometrial hyperplasia, adjacent tissues, and normal endometrial tissues by reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Sperm-associated antigen 9 concentration in serum samples from 10 healthy women, 20 women with benign diseases, and 50 women with endometrial carcinoma was detected by enzyme-linked immunosorbent assay.

Results (1) Sperm-associated antigen 9 antibodies were detected in approximately 72% of patients with endometrial cancer but not in healthy controls. (2) A significant difference has been found among pathological types and degrees (P < 0.05), and it was also found to be expressed in transferred lymph nodes. (3) Sperm-associated antigen 9 serum concentration (ng/mL) of patients with endometrial carcinoma is significantly higher than those of the healthy group (P < 0.05). Patients harboring grade 3 endometrial carcinoma were found to have significantly higher SPAG9 concentrations than those of grade 1/grade 2 (P = 0.003).

Conclusions SPAG9 is positively expressed in endometrial cancer, and with a high humoral immune response in patients. It may serve as a new type of endometrial cancer markers for early detection, diagnosis and treatment.

  • Sperm-associated antigen 9 (SPAG9)
  • Endometrial carcinoma
  • Cancer testis antigen

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  • The authors report no conflicts of interest.