Article Text

Download PDFPDF
Association of Inhibitor of Differentiation 1 Expression With Human Papillomaviruses Infections in Cervical Carcinoma
  1. Jinke Li, MD*,
  2. Lingxia Xie, MD*,
  3. Xiaoling Gan, MD*,
  4. Bao Liu, MS,
  5. Yi Zhang, PhD,
  6. Bo Song, MB,
  7. Yong Lin, PhD§ and
  8. Lina Hu, MD*
  1. *Department of Gynecology and Obstetrics,
  2. Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory,
  3. Department of Gynecological Pathology, and
  4. §Laboratory of Molecular and Translational Medicine, West China Second Hospital, Sichuan University, Chengdu, China.
  1. Address correspondence and reprint requests to Lina Hu, MD, Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, 20 Renmingnan Rd, Chengdu, Sichuan 610041, China. E-mail: cqhulina2008{at}


Background: Our recent study has shown that inhibitor of differentiation 1 (Id-1) is overexpressed in cervical carcinoma. However, the relationship between Id-1 expression and human papillomavirus (HPV) infection has not been elucidated. In this study, we investigate the association of Id-1 protein expression and HPV infection in cervical carcinoma tissues.

Methods: A total of 56 paraffin-embedded and 12 fresh cervical carcinoma tissues were collected for Id-1 and HPV detection. The Id-1 protein was detected by immunohistochemistry and Western blot in paraffin-embedded and fresh carcinoma tissues. Human papillomavirus DNA was detected and genotyped by using an oligonucleotide microarray and polymerase chain reaction.

Results: The overall HPV prevalence was 82.1%, whereas that of HPV type 16 (HPV-16) was 62.5% in cervical carcinoma. The HPV-positive samples showed higher Id-1 expression levels than the HPV-negative ones (Cochran-Mantel-Haenszel χ2 test [χ2 CMH] = 4.39, P < 0.05). The HPV-16-infected samples had higher Id-1 expression levels than the samples with infection of other single HPV genotypes (χ2 CMH = 6.42, P < 0.02). The results of Western blot were correlated to the immunohistochemistry results, showing a higher Id-1 expression level in HPV-infected especially HPV-16-infected carcinoma tissues.

Conclusions: Inhibitor of differentiation 1 expression is correlated to HPV infection in cervical carcinoma, suggesting that Id-1 plays a role in HPV-related cervical carcinogenesis.

  • Inhibitor of differentiation 1
  • Human papillomavirus
  • Cervical cancer
  • Id-1
  • HPV

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


  • Support was received through a grant from Nature Scientific Foundation of China (81001158).