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Genetic Diversity of HPV-16 E6, E7, and L1 Genes in Women With Cervical Lesions in Liaoning Province, China
  1. Zhengrong Sun, PhD,
  2. Gaowei Ren, BS,
  3. Xin Cui, BS,
  4. Weiqiang Zhou, BS,
  5. Chao Liu, BS and
  6. Qiang Ruan, MD
  1. Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.
  1. Address correspondence and reprint requests to Qiang Ruan, MD, Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 36 Sanhao St, Heping District, Shenyang, China 110004. E-mail: ruanq{at}sj-hospital.org.

Abstract

Introduction High-risk human papillomaviruses (HPVs) play a cardinal role in the etiology of cervical cancer. The most prevalent type, HPV-16, shows intratypic sequence variants that are known to differ in oncogenic potential and geographic distribution. Intratype variations in oncogenic E6/E7 and capsid L1 proteins of HPV-16 are associated with risk of viral persistence and progression.

Methods This study was designed to analyze sequence variations in E6, E7, and L1 genes of HPV-16 in patients with cervical lesion to identify the most prevalent and novel HPV-16 variants in northern China.

Results Our results showed that HPV-16 variants with respect to E6 and E7 were high prevalence of the Asian lineage: 48.3% and 51.4%, respectively. Sequences of the E6 gene revealed 4 amino acid changes of variants D25E and L83V, with 48.3% (69/143) and 11.2% (16/143), respectively, and variants H78Y and E113D in this study. The results also showed the prevalence of 4 hot spots of E7 nucleotide variations leading to N29H, N29S, and 2 silent variations, nucleotide G666A and nucleotide T846C, with 4.2% (6/142), 43% (61/142), 32.4% (46/142), and 43% (61/142), respectively. The following L1 variations were found in this study: L103F, P104K, P104Y, P104S, D105G, P106S, N108P, F109V, C172S, H228D, and T292A. It was also found that 448S was inserted and 465D was deleted in the L1 amino acid sequences of all the samples. No significant relationship between HPV-16 variants and high-grade lesions was found.

Conclusions The study provides some new data on the genetic diversity of HPV-16, which may help to understand the oncogenic potential of the virus and design the diagnosis reagents and vaccine of HPV in China. Furthermore, in-depth studies are needed to determine the clinical and biological effects of these variants.

  • HPV-16
  • E6/E7/L1 genes
  • Genetic diversity

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Footnotes

  • This work was supported by the National Natural Science Foundation of China (Grant No. 30770109).

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