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Changes in Activities of Caspase-8 and Caspase-9 in Human Cervical Malignancy
  1. Maria Themistokli Ekonomopoulou, PhD,
  2. Evaggelos Babas, PhD,
  3. Eleutheria Mioglou-Kalouptsi, PhD,
  4. Maria Malandri, MD and
  5. Zafiroula Iakovidou-Kritsi, PhD
  1. Laboratory of General Biology and Genetics, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
  1. Address correspondence and reprint requests to Zafiroula Iakovidou-Kritsi, PhD, Laboratory of General Biology and Genetics, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. E-mail: zik{at}med.auth.gr.

Abstract

Introduction The apoptotic process of programmed cell death and its dysfunctions in a variety of human diseases, including cervical cancer, has become the focus of extensive scientific research. Caspases are considered key factors in the execution of apoptosis, although there are many aspects of their role to be elucidated. It has been found that disturbance of initiator caspase-8 and caspase-9 expression or function may contribute to cancer formation/progression, and inactivation of them could promote resistance to current treatment approaches. In our research, the activities of caspase-8 and caspase-9 have been estimated during the progression of human cervical malignancy.

Materials and Methods The experimental material includes human cervical tissue samples (normal and pathological), in which enzyme activities have been measured colorimetrically.

Results Activities of caspase-8 and caspase-9 presented the highest increase, compared to the controls, in the low-grade squamous intraepithelial lesion samples (statistically significant, P < 0.01 by t test). The activities diminished in the high-grade squamous intraepithelial lesion and even more in the cancer samples but remained higher than the controls.

Conclusion The observed changes in the activities of caspase-8 and caspase-9 could be attributed to their involvement in the cervical tissue's effort to resist malignancy progression.

  • Caspase-8
  • Caspase-9
  • Cervical cancer
  • Dysplasia
  • Initiator caspases
  • Malignancy

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Footnotes

  • No funding was received for this work from any organization.

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