Article Text

Download PDFPDF
Highly Increased Maspin Expression Corresponds With Up-Regulation of miR-21 in Endometrial Cancer: A Preliminary Report
  1. Anna Torres, MD, PhD*,,
  2. Kamil Torres, MD, PhD*,,
  3. Tomasz Paszkowski, MD, PhD,
  4. Sebastian Radej, MSc, PhD*,
  5. Grzegorz J. Staśkiewicz, MD, PhD*,§,
  6. Marcello Ceccaroni, MD, PhD,,
  7. Anna Pesci, MD, PhD** and
  8. Ryszard Maciejewski, MD, PhD*,††,‡‡
  1. * Laboratory of Biostructure, Human Anatomy Department, and
  2. III Chair and Department of Gynecology, Medical University of Lublin;
  3. General and Oncologic Surgery Department, Lublin County Specialist Hospital; and
  4. § II Chair and Department of Radiology, Medical University of Lublin, Lublin, Poland;
  5. Gynecologic Oncology Division, International School of Surgical Anatomy, and
  6. Department of Obstetrics & Gynecology, European Gynaecology Endoscopy School, Sacred Heart Hospital; and
  7. ** Department of Pathology, Sacred Heart Hospital, Negrar, Verona, Italy; and
  8. †† II Chair and Department of Surgery, Medical University of Lublin; and
  9. ‡‡ Medical Emergency Department, UlTM in Rzeszów, Poland.
  1. Address correspondence and reprint requests to Anna Torres, MD, PhD, Katedra i Zakład Anatomii Prawidłowej Człowieka, Uniwersytet Medyczny w Lublinie, ul. Jaczewskiego 4, 20-094 Lublin. E-mail: anna.torres{at}; anna.torres{at}


Background: Maspin and programmed cell death 4 (Pdcd4) are tumor suppressor genes, and miR-21 is overexpressed in many solid tumors and was proven to negatively regulate a number of tumor suppressor genes including maspin and Pdcd4.

The purpose of this study was to investigate the expression of maspin, Pdcd4, and miR-21 and their interrelations with clinicopathologic features in endometrial cancer using a quantitative approach.

Methods: Maspin, Pdcd4, and miR-21 expressions were evaluated by a real-time polymerase chain reaction in 20 endometrial cancer and 10 normal endometrium samples.

Results: Maspin showed a significantly increased expression in endometrial cancer samples compared with the control group and was up-regulated by a mean factor of 46.54 (SE range, 2.367-1160.26; 95% confidence interval, 0.515-15001, P < 0.0001). Expression of miR-21 was found significantly up-regulated in the sample group in comparison to control group by a mean factor of 2.312 (SE range, 0.741-7.778; 95% confidence interval 0.191-15.0, P = 0.028). No significant differences were present in the expression level of Pdcd4 between endometrial cancer and control groups. Comparison between IA and more advanced International Federation of Gynecology and Obstetrics stages of endometrial cancer in regard to expression levels of maspin, Pdcd4, and miR-21 did not reveal any significant differences. Similarly, no differences were encountered when histopathologic grading, myometrial invasion, age, body mass index, and parity were taken into consideration.

Conclusions: Association between increased maspin expression and up-regulation of miR-21 in endometrial cancer suggests distinct and tissue-specific relationships of the 2 molecules in this type of malignancy and requires further studies that would reveal its clinical relevance.

  • Endometrial cancer
  • Maspin
  • PDCD4
  • miR-21

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


  • The authors declare that they have no competing interests.