Article Text

PDF
Survivin and Telomerase Expression in the Uterine Cervix of Women With Human Papillomavirus-Induced Lesions
  1. Lyliana Coutinho Resende Barbosa, MD, DHSc*,
  2. Ismael Dale Cotrim Guerreiro da Silva, MD, DSc,
  3. José Carlos Corrêa, MD, MSc and
  4. Julisa Chamorro Lascasas Ribalta, MD, DSc
  1. * Gynecologic Oncology Services, Universidade do Vale do Sapucaí (UNIVÁS), Pouso Alegre, MG;
  2. Department of Gynecology, Universidade Federal de São Paulo (UNIFESP-EPM), São Paulo, SP; and
  3. Institute of Pathology José Carlos Corrêa, Pouso Alegre, MG,Brazil.
  1. Address correspondence and reprint requests to Lyliana Coutinho Resende Barbosa, MD, DHSc, Av Alfredo Custódio de Paula 490, Medicina, CEP 37550-000 Pouso Alegre, MG, Brazil. E-mail: lylianacbarbosa{at}oi.com.br.

Abstract

Introduction: Infection by human papillomavirus is the most important risk factor in the pathogenesis of uterine cervical cancer. The aims of this study were to evaluate the expression of survivin protein and telomerase enzyme in samples of uterine cervix from women with human papillomavirus-induced lesions and to determine the relationship between survivin and telomerase expression and the different grades of cervical squamous intraepithelial neoplasia and invasive cervical carcinoma.

Methods: Biopsy samples from the uterine cervix of 105 women aged 18 to 80 years were analyzed. The patients were divided into 5 groups: WN group, 20 patients without neoplasia; CIN-1 group, 24 patients with grade 1 cervical intraepithelial neoplasia (CIN), grade 1; CIN-2 group, 20 patients with CIN grade 2; CIN-3 group, 24 patients with CIN, grade 3; and ICC group, 17 patients with invasive cervical carcinoma. Human papillomavirus detection, telomerase activity, and survivin expression were assessed using polymerase chain reaction (PCR), real-time PCR (RT-PCR), and immunochemistry, respectively.

Results: There was a significant increase in the expression of telomerase and survivin associated with the severity of the lesion.

Conclusions: The results suggest that mechanisms that promote both cell proliferation (telomerase activity) and cell survival (survivin expression) are active in cervical cancer and its precursor lesions. There was a negative correlation between survivin expression and the number of PCR cycles necessary to detect telomerase activity in the total sample, achieving statistical significance in patients in the CIN-3 group.

  • Telomerase
  • Survivin protein
  • Human papillomavirus
  • Uterine cervical cancer
  • Cervical intraepithelial neoplasia

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.