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Overexpression of Bmi-1 in Uterine Cervical Cancer: Correlation With Clinicopathology and Prognosis
  1. Xin Zhang, PhD*,
  2. Chuan-Xin Wang, MD, PhD*,
  3. Cheng-bao Zhu, MA Sc,
  4. Jian Zhang, PhD*,
  5. Shi-feng Kan, MA Sc*,
  6. Lu-tao Du, MA Sc*,
  7. Wei Li, PhD*,
  8. Li-li Wang, PhD* and
  9. Shun Wang, MA Sc*
  1. * Department of Clinical Laboratory, Qilu Hospital, Shandong University; and
  2. Department of Clinical Laboratory, Jinan Infectious Disease Hospital, Jinan, China.
  1. Address correspondence and reprint requests to Chuan-Xin Wang, MD, PhD, Department of Clinical Laboratory, Qilu Hospital, Shandong University, 107 Wenhua Rd, Jinan 250012, China. E-mail: cxwang{at}


Introduction: B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) is a member of polycomb group, which participates in axial patterning, hematopoiesis, cell cycle regulation, and senescence. Recently, overexpression of Bmi-1 has been reported in various human cancers and proved to be associated with poor survival. The aim of this study was to investigate the expression of Bmi-1 protein in human uterine cervical cancer (UCC) and explore its associations with clinicopathological factors and prognosis.

Methods: Western blot was used to detect the expression of Bmi-1 in 4 human cervical cancer cell lines (Hela, SiHa, CasKi, and C33A) and a normal cervical epithelial cell line. In addition, 152 UCC and 30 adjacent normal cervical paraffin-embedded samples were collected to detect Bmi-1 expression by immunohistochemistry.

Results: Western blot analysis showed Bmi-1 was overexpressed in 4 human UCC cell lines but not in the normal cervical epithelial cell line. Moreover, immunohistochemical staining revealed Bmi-1 was overexpressed in 63.2% UCC tissues (Bmi-1 ++ or +++), and the overexpression of Bmi-1 protein was significantly correlated with tumor size (P = 0.046), clinical stage (P = 0.021), and regional lymph nodes metastasis (P = 0.010). Survival analysis showed a significant difference between Bmi-1 protein overexpression and poor survival (P = 0.021). Cox proportional hazards risk analysis indicated that Bmi-1 protein overexpression was an independent prognostic factor for overall survival.

Conclusions: B-cell-specific Moloney murine leukemia virus integration site 1 is overexpressed in UCC and correlated with adverse clinical characteristics and poor prognosis, which suggests that the Bmi-1 might participate in the development and progression of UCC and have clinical potential not only as a useful predictor of aggressive phenotype but also a promising prognostic predictor.

  • Polycomb group
  • Bmi-1
  • Western blot
  • Immunohistochemistry
  • Uterine cervical cancer
  • Prognosis

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