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Association Between the CDC6 G1321A Polymorphism and the Risk of Cervical Cancer
  1. Xing-Dong Xiong, PhD*,,
  2. Li-Qin Zeng, MD,
  3. Qing-Yuan Xiong, BSc,
  4. Sheng-Xiang Lu, BSc,
  5. Zhi-Zhen Zhang, PhD*,,
  6. Xi-Ping Luo, MD and
  7. Xin-Guang Liu, PhD*,
  1. * Department of Biochemistry and Molecular Biology, and
  2. Aging Institute, Guangdong Medical College, Dongguan; and
  3. Department of Gynecology, Guangdong Women and Children Hospital and Health Institute, Guangzhou, China.
  1. Address correspondence and reprint requests to Xin-Guang Liu, PhD, Department of Biochemistry and Molecular Biology, Aging Institute, Guangdong Medical College, Dongguan 523808, China. E-mail: xgliu64{at}126.com.

Abstract

Introduction: Cell division cycle protein 6 (CDC6) plays critical roles in DNA replication and carcinogenesis. The biological significance of the CDC6 G1321A polymorphism (V441I, rs13706) on cervical carcinogenesis is still unknown. Here, we examined the potential influence of this polymorphism on cell proliferation and the individual's susceptibility to cervical cancer.

Methods: We genotyped the CDC6 G1321A polymorphism in 87 cervical cancer cases and 110 healthy female subjects. Unconditional logistic regression analysis was used to estimate the association between the genotypes and the risk of cervical cancer. The BrdU incorporation assay was applied to analyze the effect of this polymorphism on cell proliferation.

Results: Compared with the GG homozygotes, the cervical cancer risk was significantly reduced in the individuals with the heterozygous AG genotype (odds ratio [OR], 0.53; 95% confidence interval [CI], 0.28-0.98; P = 0.042) or the homozygous AA genotype (OR, 0.29; 95% CI, 0.09-0.89; P = 0.030). Further stratified analyses showed that the decreased risk of cervical cancer was more evident among younger subjects (≤44 years old) with the AG or AA genotypes (OR, 0.44; 95% CI, 0.21-0.92; P = 0.029 and OR, 0.12; 95% CI, 0.03-0.61; P = 0.010, respectively). The BrdU incorporation assay showed that 293T cells transfected with CDC6-441I (1321A) had a lower proliferation rate in comparison with those transfected with CDC6-441V (1321G), although the difference did not reach statistical significance at the 0.05 level.

Conclusions: The CDC6 G1321A polymorphism may contribute to the risk of cervical cancer. Further studies with more subjects and in diverse ethnic populations are necessary to confirm the general validity of our findings.

  • CDC6
  • Single nucleotide polymorphism
  • Cervical cancer
  • Risk

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Footnotes

  • The authors declare that there are no conflicts of interest.

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