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Basic Sciences
Molecular Variants of HPV Type 16 E6 Among Honduran Women
  1. Nelba Tabora*,
  2. Willem J.G. Melchers†,
  3. Leen-Jan Van Doorn‡,
  4. Wim Quint‡ and
  5. Annabelle Ferrera, PhD*
  1. *School of Microbiology, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras; and
  2. †Department of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen; and
  3. ‡DDL Diagnostic Laboratory. Voorburg, The Netherlands.
  1. Address correspondence and reprint requests to Annabelle Ferrera, PhD, School of Microbiology, Universidad Nacional Autónoma de Honduras, P.O. Box 30078, Tegucigalpa, Honduras. E-mail: f_annabelle{at}hotmail.com; annabelle{at}amnettgu.com.

Abstract

Objective: In this study, we investigated the prevalence of human papillomavirus 16 (HPV-16) variants in Honduran women with normal cytology and with dysplasia and cervical cancer.

Methods: Samples identified as positive for HPV-16 by SPF10-LiPA were tested for intratypic subtypes and variants by analysis of the E6/E7 region using a novel reverse hybridization assay (line probe assay).

Results: We found that most infections in all clinical groups belong to the E6 European variants, suggesting that HPV-16 non-European variants do not represent an additional factor associated with increased occurrence of high-grade cervical lesions in the studied population. Among the 106 HPV-16-positive women analyzed, E-350G was the most prevalent variant in all different disease stages, being present in 18% of cervical cancer, 13% of cervical intraepithelial neoplasia grade III (CIN III), 5% of CIN II, 5% of CIN I, and 20% of control samples. Mixed variants of HPV-16 infections were detected in 7.7% of the samples, mostly in women with normal cytology.

Conclusions: This study shows for the first time the diversity of HPV-16 variants in cervical samples of Honduran women.

  • Human papillomavirus
  • HPV-16 variants
  • Honduras

https://doi.org/10.1111/IGC.0b013e3181d1c2f8

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