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Down-Regulation of HLA Class I Antigen in Human Papillomavirus Type 16 E7 Expressing HaCaT Cells: Correlate With TAP-1 Expression
  1. Wei Li, PhD*,
  2. Xiao-Mei Deng, PhD*,
  3. Chuan-Xin Wang, MD, PhD*,
  4. Xiao Zhang, PhD*,
  5. Gui-Xi Zheng, PhD*,
  6. Jian Zhang, PhD* and
  7. Jin-Bo Feng, PhD
  1. *Department of Clinical Laboratory, and
  2. The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital, Shandong University, Jinan, China.
  1. Address correspondence and reprint requests to Chuan-Xin Wang, MD, PhD, Department of Clinical Laboratory, Qilu Hospital, Shandong University, 107 Wenhua Rd, Jinan 250012, China. E-mail: wcx6601{at}126.com.

Abstract

Objectives: High-risk human papillomaviruses (HPVs) are the major causative agents of cervical cancer, and the E6 and E7 genes encode the major HPV oncoproteins. The E7 protein of high-risk HPV types disturbs cell cycle control and down-regulates components of the antigen presentation pathway, suggesting a role for E7 in tumor immune evasion. We previously reported that HPV-16 E7 expression and down-regulation of HLA class I was highly correlated in cervical lesions. This study was aimed to determine whether HPV-16 E7 oncoprotein could down-regulate surface HLA class I antigen in HPV-16 E7-transfected cells, and whether it had correlation with the expression of the transporter associated with antigen processing (TAP).

Methods: The HPV-16 E7 open reading frame was transfected into HaCaT cells. After G418 selection, resistant colonies were individually picked and expanded into clonal cell lines. Using the fluoresence-activated cell sorting analysis, the levels of cell surface HLA class I antigen and intracellular TAP-1 and TAP-2 expressions were detected.

Results Compared with the empty vector control, a statistical significant decrease of approximately 50% in cell surface HLA class I expression was observed in HPV-16 E7 expressing HaCaT cells (P < 0.001). Moreover, the expression of HPV-16 E7 in HaCaT cells resulted in decreased expression of TAP-1 that was essential for HLA class I expression at the cell surface, a statistical significant decrease of approximately 40% compared with that with the empty vector control (P < 0.001).

Conclusions: Our finding demonstrates that HPV-16 E7 down-regulates surface HLA class I antigen, which in part correlates with the decrease of TAP-1.

  • HPV-16 E7
  • HLA class I
  • TAP
  • Cervical cancer
  • HaCaT cell

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Footnotes

  • This work was supported by the grants from the National Natural Science Foundation Projects (No. 30672010).

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