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Proteomics-Based Approach Identified Differentially Expressed Proteins With Potential Roles in Endometrial Carcinoma
  1. Zhengyu Li, MD*,,
  2. Wenjiao Min, MM,
  3. Canhua Huang, PhD,
  4. Shujun Bai, PhM,
  5. Minghai Tang, PhM and
  6. Xia Zhao, MD§
  1. *Department of Gynecology and Obstetrics, West China Second Hospital;
  2. State Key Laboratory of Biotherapy, West China Hospital, Sichuan University;
  3. The 4th People's Hospital of Chengdu; and
  4. §Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China.
  1. Address correspondence and reprint requests to Xia Zhao, MD, Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu 610041, People's Republic of China. E-mail: qingshanxiagu{at}tom.com.

Abstract

Instruction: We used proteomic approaches to identify altered expressed proteins in endometrial carcinoma, with the aim of discovering potential biomarkers or therapeutic targets for endometrial carcinoma.

Methods: The global proteins extracted from endometrial carcinoma and normal endometrial tissues were separated by 2-dimensional electrophoresis and analyzed with PDQuest (Bio-Rad, Hercules, Calif) software. The differentially expressed spots were identified by mass spectrometry and searched against NCBInr protein database. Those proteins with potential roles were confirmed by Western blotting and immunohistochemical assays.

Results: Ninety-nine proteins were identified by mass spectrometry, and a cluster diagram analysis indicated that these proteins were involved in metabolism, cell transformation, protein folding, translation and modification, proliferation and apoptosis, signal transduction, cytoskeleton, and so on. In confirmatory immunoblotting and immunohistochemical analyses, overexpressions of epidermal fatty acid-binding protein, calcyphosine, and cyclophilin A were also observed in endometrial carcinoma tissues, which were consistent with the proteomic results.

Conclusions: Our results suggested that these identified proteins, including epidermal fatty acid-binding protein, calcyphosine, and cyclophilin A, might be of potential values in the studies of endometrial carcinogenesis or investigations of diagnostic biomarkers or treatment targets for endometrial carcinoma.

  • Endometrial carcinoma
  • Proteomics
  • 2-Dimensional electrophoresis
  • Biomarker

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Footnotes

  • This work is a project of the National Natural Science Foundation of China and a National 863 High-Tech Research Development project, which is supported by the National Key Basic Research Program of China (30070835 and 30070294). Zhengyu Li and Wenjiao Min both contributed equally to this work. There is no conflict of interests.

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