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Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary
  1. Yi Miao, MD*,
  2. Bin Cai, PhD*,
  3. Ling Liu, PhD*,,
  4. Yixia Yang, MD, PhD* and
  5. Xiaoping Wan, MD, PhD*
  1. * Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, People's Republic of China; and
  2. Departments of Medicine and Medical Biophysics, University of Toronto, Toronto, Canada.
  1. Address correspondence and reprint requests to Xiao-ping Wan, MD, PhD, Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, 85 Wujin Rd, Shanghai, 200080, People's Republic of China. E-mail: wanxiaoping61{at}


Objective: To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.

Methods: Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.

Results: Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.

Conclusions: The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.

Abbreviations: CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β

  • Clear cell carcinoma of the ovary
  • Gene expression profiling
  • Annexin IV

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