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Clinical Significance of Human Papillomavirus Genotype by Linear Array Assay in Japanese Women With Uterine Cervical Lesions and Type 16 Physical Status by In Situ Hybridization
  1. Mizuho Futai, MD*,
  2. Jun Watanabe, MD, PhD,
  3. Toshiko Jobo, MD, PhD*,
  4. Shinpei Tsunoda, MD, PhD*,
  5. Yukari Nishimura, PhD,
  6. Kayoko Watanabe, MS,
  7. Isao Okayasu, MD, PhD and
  8. Nobuya Unno, MD, PhD*
  1. *Departments of Obstetrics and Gynecology, and
  2. Departments of Pathology, School of Medicine, Kitasato University, Sagamihara, Kanagawa, Japan; and
  3. Departments of Roche Diagnostics Japan, Tokyo, Japan.
  1. Address correspondence and reprint requests to Mizuho Futai, MD, Department of Obstetrics and Gynecology, School of Medicine, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan. E-mail: futai{at}


The aim of this study was to clarify the relation of human papillomavirus (HPV) genotypes and physical status in the cervical neoplasm of Japanese patients with the grade of the disease.

Human papillomavirus genotype was detected using a linear array genotyping assay. Human papillomavirus status, diffuse or punctate signal pattern, was studied by biotynyl-tyramide-based in situ hybridization for positive cases of HPV-16.

Human papillomavirus types 16, 52, 58, and 31, in descending order of frequency, were prevalent. The rates of HPV infection in patients with cervical intraepithelial neoplasia (CIN) or squamous cell carcinoma (SCC) were significantly higher than those in patients without cervical lesions. The frequency of HPV single infection in SCC was higher than that in CIN1 or CIN2. In an unspecified-risk HPV, types 66 and 70 were found in SCC and 62, 71, and 82 were detected in CIN3. The diffuse pattern was more frequent in CIN, and the punctate pattern was more frequent in SCC.

Human papillomavirus types 16, 52, 58, and 31 were frequently detected in Japanese women with cervical neoplasias, and several unspecified-risk HPVs might be high-risk types. A single infection of HPV and a punctate signal pattern seemed to be closely correlated with cervical carcinogenesis.

  • HPV genotype
  • Linear array hybridization
  • In situ hybridization
  • Cervical intraepithelial neoplasia
  • Carcinoma

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  • This study was supported by a grant-in-aid (NO. 20791161) for scientific research from the Ministry of Education, Culture, Sport, Sciences and Technology, Japan.