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Association of TNFA (−308G>A) and IL-10 (−819C>T) Promoter Polymorphisms With Risk of Cervical Cancer
  1. HariOm Singh, MSc*,
  2. Meenu Jain, PhD*,
  3. Rekha Sachan, MS and
  4. Balraj Mittal, PhD*
  1. *Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences;
  2. Department of Obstetrics and Gynecology, CSMMU, Lucknow, India.
  1. Address correspondence and reprint requests to B. Mittal, MD, Department of Genetics, SGPGIMS, Lucknow-226014, India. E-mail: .balraj{at}sgpgi.ac.in; bml_pgi{at}yahoo.com.

Abstract

Etiology of cervical cancer is associated with excessive inflammation mediated tumorigenesis. Pro and anti-inflammatory cytokines (tumor necrosis factor α, TNFA and interleukin, IL-10) are involved in fighting against the tumorigenesis. Therefore, the present study was designed to evaluate the association of TNFA (−308G>A) and IL-10 (−819C>T) gene polymorphism with risk of cervical cancer. One hundred fifty histopathologically confirmed patients with cervical cancer and 162 age, ethnically-matched cervical cytology negative healthy controls were genotyped for TNFA (−308 G>A) and IL-10 (−819 C>T) polymorphisms using PCR-RFLP. Individuals with combination of TNFA -308GA+AA genotype and A allele were at elevated risk of cervical cancer (odds ratio (OR) = 2.24; P = 0.018 and OR, 2.05; P = 0.012). Frequency of IL-10 −819CT+TT genotype combination and T allele was slightly higher in cases as compared with controls but difference was not significant (OR = 1.52; P = 0.069 and OR = 1.38; P = 0.051). In association of genotypes with clinical characteristics, presence of TNFA −308GA+AA genotype conferred high risk for the stages (IB) (OR = 2.86, P = 0.039) and stages (III) (OR = 2.52; P = 0.015) of cervical cancer. In contrast, IL-10 -819TT genotype was not associated with higher risk of clinical characteristics of cervical cancer. In conclusion, individuals with TNFA −308*A allele carriers were at significantly higher risk of cervical cancer particularly early (IB) and advanced stages (III). However, IL-10 (−819C>T) polymorphism was not associated with risk of cervical cancer.

  • Genetic polymorphism
  • TNFA
  • Interleukin-10
  • Cervical cancer

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Footnotes

  • The study was supported by research grant from UP Council of Science and Technology, UPCST, India.

  • Conflict of interest: None

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