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Experimental Research on the Depressant Effect of Aspirin on Ishikawa Adenocarcinoma Endometrium Cell Growth
  1. Ma Xiaoxin, PhD,
  2. Jin Yingnan, MS,
  3. Li Yanxia, MS,
  4. Li Shu, MS,
  5. He Yuanqi, MS and
  6. Lv Hongwei, MS
  1. Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, China.
  1. Address correspondence and reprint requests to Ma Xiaoxin, PhD, Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, China 110004. E-mail: maxiaoxin666{at}yahoo.com.cn.

Abstract

Objective: To investigate the effect of aspirin on human Ishikawa adenocarcinoma endometrium cell proliferation and apoptosis and its related mechanism through in vitro experiments.

Methods: The effects on Ishikawa adenocarcinoma endometrium cell proliferation and cell cycle of aspirin at different intervals and concentrations were determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and flow cytometry; cell morphous change after the effect of aspirin was observed with transmission electron microscope; and the effect of aspirin on B-cell lymphoma/leukemia-x, long (Bcl-xl) proteinum expression was determined with Western blot.

Results: Aspirin had a significant depressant effect on human Ishikawa adenocarcinoma endometrium cell proliferation, and the effect showed time and dose dependence (P < 0.05). Aspirin-induced cell blockage at G1 phase, elevated cell apoptosis rate, and its effect were related with its concentration (P < 0.05). After treatment, cell volume was reduced, chromatin was seen concentrated and aggregated around the edge of nuclear membrane, and apoptotic body was formed. Aspirin decreased Bcl-xl proteinum expression, and the effect was related with its concentration (P < 0.05).

Conclusions: Aspirin has a distinct depressant effect on human Ishikawa adenocarcinoma endometrium cell growth, and its effect may be realized by lowering Bcl-xl proteinum expression.

  • Aspirin
  • Ishikawa cell
  • Bcl-xl proteinum

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Footnotes

  • This project was funded by the College Science Technology Research Project of the Education Department, Liaoning (grant No. 05L477).

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