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Regulation of Ovarian Cancer Cell Adhesion and Invasion by Chloride Channels
  1. Min Li*,
  2. Qing Wang,
  3. Wei Lin* and
  4. Bo Wang*
  1. *Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong; and
  2. Department of Obstetrics and Gynecology, Anhui Provincial Hospital, Hefei, PR China.
  1. Address correspondence and reprint requests to Bo Wang, Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, PR China. E-mail: wangboshandong{at}


The purpose of this study was to investigate the effects of chloride (Cl) channels on the adhesive and invasive potentials of human ovarian cancer cell line A2780. By using the adhesion and Transwell invasion assays, we showed that 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (200 μmol/L), a nonselective Cl channel blocker, significantly inhibits cancer cell adhesion and invasion. 5-Nitro-2-(3-phenylpropylamino)-benzoate (200 μmol/L), niflumic acid (100 μmol/L), and tamoxifen (30 μmol/L) had similar inhibitory effects. Regulatory volume decrease is markedly suppressed by 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid, 5-nitro-2-(3-phenylpropylamino)-benzoate, niflumic acid, and tamoxifen. Moreover, intracellular calcium concentration ([Ca2+]i) measurements indicated that a Cl channel-mediated increase in [Ca2+]i is also one of the mechanisms of cancer cell adhesion and invasion. Our results strongly suggest that Cl channels may regulate ovarian cancer cell adhesion and invasion, probably through inducing a regulatory volume decrease and mediating a [Ca2+]i increase.

  • Chloride channels
  • Cell adhesion
  • Cell invasion
  • Ovarian cancer cells

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