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Comparison of Human Papillomavirus Detection and Typing by Hybrid Capture 2, Linear Array, DNA Chip, and Cycle Sequencing in Cervical Swab Samples
  1. Jae Kwan Lee, MD*,
  2. Mi Kyung Kim, PhD,
  3. Seung Hun Song, MD*,
  4. Jin Hwa Hong, MD*,
  5. Kyung Jin Min, MD*,
  6. Jae Hoon Kim, MD,
  7. Eun-Seop Song, MD§,
  8. Jungpil Lee, MD,
  9. Jong-Min Lee, MD and
  10. Soo-Young Hur, MD**
  1. * Department of Obstetrics and Gynecology, Korea University College of Medicine;
  2. Division of Basic Sciences, Carcinogenesis Branch, National Cancer Center;
  3. Yongdong Severance Hospital, Yonsei University College of Medicine;
  4. § College of Medicine, Inha University;
  5. Ajou University School of Medicine;
  6. East-West Neo Medical Center, Kyung Hee University;
  7. ** Holy Family Hospital, College of Medicine, The Catholic University of Korea, Korea.
  1. Address correspondence and reprint requests to Mi Kyung Kim, PhD, CancerEpidemiology Branch, Division of Cancer Epidemiology and Management, National Cancer Center, 809 Madu-dong, Ilsan-gu, Goyang-si, Gyeonggi-do 411-769, Korea. E-mail: alrud{at}


Although the Hybrid Capture II (HC II) assay can detect 13 high-risk human papillomavirus (HPVs), it does not yield any genotype-specific information. We evaluated the performance of 4 HPV DNA tests, namely, HC II, Linear Array (LA), DNA chip, and cycle sequencing for their capacity to detect the presence of high-risk HPV DNA and HPV-associated cervical lesions. Seventy-six women who were referred to the colposcopy clinic for abnormal cytology were enrolled. The women were examined using liquid-based cytology, colposcopy-directed biopsy, and HPV DNA tests. After DNA extraction from a single sample, HPV DNA tests were performed by all 4 methods on the same specimen. The LA test has higher HPV-positive rates than HC II for cervical intraepithelial neoplasia I (83.3% vs 61.1%; P < 0.01) and for cervical intraepithelial neoplasia II and more severe lesions (100.0% vs 80.0%; P < 0.01). The concordance between the DNA chip and LA tests was 89.5%, confirming substantial agreement (κ coefficient = 0.73), and the concordance between HC II and the DNA chip was 80.3%, also showing substantial agreement (κ coefficient = 0.738). The concordance for 15 high-risk HPV genotypes between LA and sequencing was 82.5% with a κ value of 0.536. Furthermore, the LA test was more sensitive in the detection of high-grade cervical lesions than HC II (100% vs 92.3%, P < 0.01). The LA test showed superior sensitivity in the detection of clinically relevant HPV infections and has proven to be an accurate tool for identifying individual HPV types, especially in cases of multiple HPV infections.

  • HPV DNA test
  • Cycle sequencing
  • Linear Array
  • HPV DNA chip
  • Hybrid Capture II

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