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Junctional Adhesion Molecule: An Expression in Human Endometrial Carcinoma
  1. Hisato Koshiba, MD, PhD*,
  2. Kenichi Hosokawa, MD, PhD*,
  3. Akiko Kubo,
  4. Norimasa Tokumitsu,
  5. Ai Watanabe, MD, PhD* and
  6. Hideo Honjo, MD, PhD*
  1. * Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kamigyoku, Kyoto;
  2. Department of Hospital Pharmacology, Kyoto Pharmaceutical University, Yamashinaku, Kyoto; and
  3. Tokyo Scientific Laboratory, Shinjukuku, Tokyo, Japan.
  1. Address correspondence and reprint requests to Hisato Koshiba, Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kamigyoku, Kyoto 602-8566, Japan. E-mail: hkoshiba{at}


Junctional adhesion molecule A (JAM-A) is involved in cell-cell contact and tight junction formation. Loss of cell adhesion molecules may be associated with high histologic grade and invasiveness of endometrial carcinoma. We attempted to determine JAM-A expression in human endometrial carcinoma and its correlations with pathologic features, stage, and survival. Junctional adhesion molecule A expression in human endometrial carcinoma was evaluated by immunohistochemistry. In addition, we cultured human well and poorly differentiated endometrial adenocarcinoma cell lines, Ishikawa cells, and KLE in 3-dimensional basement membrane preparation, and JAM-A expression in these cells was assessed by real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Junctional adhesion molecule A immunostaining intensity was negatively correlated with histologic grade (τ = −0.420, P < 0.0001), myometrial invasion (τ = −0.306, P < 0.01), and stage (τ = −0.383, P < 0.0001). Low JAM-A immunostaining intensity was associated with positive vascular space involvement (P < 0.01). Moreover, low immunostain intensity was significantly (P < 0.0001) related to low overall survival rate and progression-free survival rate. Additionally, in our 3-dimensional epithelial cell culture, JAM-A expression in poorly differentiated adenocarcinoma was significantly lower than that in well-differentiated adenocarcinoma (P < 0.001). Junctional adhesion molecule A expression seems to be reduced in high-grade or advanced endometrial carcinoma and may be a prognostic factor.

  • Junctional adhesion molecule A
  • Endometrial carcinoma
  • Histologic grade
  • Prognostic factor
  • 3-dimensional culture

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