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ORIGINAL ARTICLE
High-intensity electric pulses induce mitochondria-dependent apoptosis in ovarian cancer xenograft mice
  1. C. Li*,
  2. L.-N. Hu†,
  3. X.-J. Dong*,
  4. C.-X. Sun‡ and
  5. Y. Mi‡
  1. * Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China;
  2. † Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Sichuan University, Chengdu, China; and
  3. ‡ The Key Lab of High Engineering and Electrical New Technology, College of Electrical Engineering, Chongqing University, Chongqing, China
  1. Address correspondence and reprint requests to: Li Na Hu, MD, Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Sichuan University, 37 Guoxue Road, Chengdu 610081, China. Email: lcxmm{at}yahoo.com.cn

Abstract

Human ovarian cancer models were established in nude mice by transplanting SKOV3 cells, and then tumors were exposed to high-intensity electric pulses with a voltage 1000 V, frequency of 1000 Hz, and duration of 250 ns for 1 min. Mitochondria permeability transition pore (PTP) was inspected by cofocal microscope; cytochrome C (Cyt C) and apoptosis-induced factor (AIF) were determined by immunohistochemistry; and voltage-dependent anion channel (VDAC) was measured by immunofluorescence. High-intensity electric pulses exposure led to increases of PTP, Cyt C, and AIF and a decrease of VDAC. These findings revealed that high-intensity electric pulses activated mitochondria electroporation, apoptosis was realized via mitochondria pathway.

  • apoptosis
  • electric pulse
  • mitochondria
  • ovarian cancer

https://doi.org/10.1111/j.1525-1438.2007.01182.x

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