Article Text

Download PDFPDF
LINE- 1 hypomethylation level as a potential prognostic factor for epithelial ovarian cancer
  1. J. Pattamadilok*,
  2. N. Huapai,
  3. P. Rattanatanyong,
  4. A. Vasurattana*,
  5. S. Triratanachat*,
  6. D. Tresukosol* and
  7. A. Mutirangura
  1. *Department of Obstetrics and Gynecology and
  2. Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  1. Address correspondence and reprint requests to: Apiwat Mutirangura, MD, PhD, Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. Email: mapiwat{at}chula.ac.th

Abstract

A genome-wide hypomethylation is a common and crucial event in cancer. This study was to evaluate common epithelial ovarian cancer (EOC) if long interspersed element-1 (LINE-1) repetitive sequences methylation levels are progressively decreased during multistage carcinogenesis and there are the correlation between LINE-1 methylation levels and clinicopathologic characteristics. A total of 59 pairs of microdissected EOC tissues obtained from patients with EOC were examined for the methylation levels of LINE-1 repetitive sequences by a COBRALINE-1 (combined bisulfite restriction analysis of LINE-1) PCR protocol. The methylation levels were correlated with clinicopathologic parameters to determine the potential role of global hypomethylation as a prognostic marker for EOC. The LINE-1 methylation levels of 59 EOCs, 34.87 ± 7.39%, were lower than in representative normal ovarian tissues (46.89 ± 8.31%; 95% CI: 9.42–14.62; P< 0.001, paired-two-tailed t test). A decrease in the LINE-1 level of methylation was correlated with histological subtypes, higher FIGO and advanced tumor grade. Patients with greater hypomethylation (i.e., a methylation level ≤ 34.87%) had poorer mean overall survival (P= 0.003) and a lower mean progression-free interval (P< 0.001). Therefore, progressive decrease in LINE-1 methylation level is a common and important epigenetic process in ovarian multistep carcinogenesis. Moreover, the COBRALINE-1 method has the potential to be used as a tumor marker for EOC.

  • COBRALINE-1
  • epithelial ovarian cancer
  • global hypomethylation
  • LINE-1 methylation
  • tumor marker

Statistics from Altmetric.com

Footnotes

  • J. Pattamadilok is presently at Chulabhorn Cancer Centre, Chulabhorn Research Institute, Bangkok, Thailand.

  • N. Huapai is presently at Mathayom Watsrichanpradit School, Samutprakarn, Thailand.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.