Article Text

Download PDFPDF
Mutation analysis of the tumor suppressor gene PPP2R1B in human cervical cancer
  1. L.-S. Yeh*,
  2. Y.-Y. Hsieh*,,
  3. J.-G. Chang,
  4. W.W.-C. Chang§,
  5. C.-C. Chang* and
  6. F.-J. Tsai
  1. *Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan;
  2. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; and Departments of
  3. Molecular Medicine,
  4. §Otolaryngology; and
  5. Pediatrics and Medical Genetics, China Medical University Hospital, Taichung, Taiwan
  1. Address correspondence and reprint requests to: Fuu-Jen Tsai, MD, PhD, Department of Pediatrics and Medical Genetics, China Medical University Hospital, No. 2 Yuh-Der Road, Taichung, Taiwan. Email: d0704{at}www.cmuh.org.tw

Abstract

Protein phosphatase 2A (PP2A) holoenzyme plays a critical role in cell cycle control and growth factor signaling. The PPP2R1Bgene encodes the beta isoforms of the subunit A of the PP2A. We aimed to evaluate the role of the PPP2R1Bgene in the pathogenesis of cervical cancer. Twenty-four women with primary cervical cancer were included. All resected specimens were divided into two groups: (1) cervical cancers (n= 24), (2) nearby noncancerous tissues (n= 24). We performed nested reverse transcriptase–polymerase chain reaction analysis and complementary DNA sequencing on the genomic DNA samples of all specimens. The aberrant transcripts and gene mutation as well as the genotype and allele frequencies of codon 66 CTA/CTG of PPP2R1Bgenes in both groups were compared. The percentages of aberrant transcripts between both groups were nonsignificantly different (20.8% vs 33.3%). There was no mutation in all specimens. The genotype and allele frequencies between both groups were non-different. Proportions of CTA homozygote/heterozygote/CTG homozygote were (1) 66.7/8.3/25% and (2) 58.3/12.5/29.2%. Proportions of CTA/CTG alleles in both groups were (1) 70.8/29.2% and (2) 64.6/35.4%. We conclude that PPP2R1Bgenes may not play a role in the carcinogenesis of cervical cancer. Mutations of PPP2R1Bgene are not frequent in cervical cancer.

  • cervical cancer
  • loss of heterozygosity
  • PPP2R1B
  • protein phosphatase 2A
  • tumor suppressor gene

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.