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Concurrent high expression of human telomerase reverse transcriptase and human nonmetastatic clone 23 in high-grade squamous intraepithelial neoplasia and squamous cell carcinoma of uterine cervix
  1. C.-G. Hsu1,*,,
  2. P.-H. Wang1,,
  3. J.-L. Ko§,
  4. G.-D. Chen,
  5. H. Chang,
  6. S.-F. Yang*,
  7. S.-C. Chen# and
  8. L.-Y. Lin
  1. *Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;
  2. Department of surgery, Da Chien General Hospital, Miao-Li, Taiwan;
  3. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan;
  4. §Institute of Medical and Molecular Toxicology, Chung Shan Medical University, Taichung, Taiwan;
  5. School of Medicine, Chung Shan Medical University, Taichung, Taiwan;
  6. Department of Pathology, Chung Shan Medical University, Taichung, Taiwan;
  7. #Department of Family Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan
  1. Address correspondence and reprint requests to: Long-Yau Lin, MD, PhD, School of Medicine, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung 40201, Taiwan. Email: ming84921345{at}yahoo.com.tw

Abstract

Human telomerase reverse transcriptase (hTERT) and human nonmetastatic clone 23 (nm23-H1) may be separately involved in tumor progression of uterine cervix. We therefore investigate the correlations of hTERT and nm23-H1 in cervical carcinogenesis and further check their application. One hundred and twenty-eight cervical tissues, including 48 squamous cell carcinoma (SCC), 36 high-grade cervical intraepithelial neoplasia (CIN) (CIN 2 and CIN 3), 20 low-grade CIN 1, and 24 normal cases, were collected for immunohistochemical expression of hTERT and nm23-H1. Spearman rank correlation analysis was applied to assess their correlation in these samples. The Fisher exact or Chi-square test was used to evaluate the expression of hTERT or nm23-H1 among each subgroup. The sensitivity, specificity, positive and negative predictive values (PPV and NPV), and accuracy of hTERT and/or nm23-H1 were calculated for the prediction of high-grade CIN and SCC. We found normal cervix and CIN 1 samples had concurrent low expression of hTERT and nm23-H1, whereas high-grade CIN and SCC samples had concurrent high immunoreactivities. The hTERT alone and hTERT or nm23-H1 in combination had better sensitivity, NPV, and accuracy. The nm23-H1 alone as well as hTERT and nm23-H1 in combination had better specificity and PPV. Our results reveal a significantly positive relationship between expression of hTERT and nm23-H1 in normal and neoplastic tissues of uterine cervix. We suggest high expression of hTERT alone and hTERT or nm23-H1 in combination can be offered additional molecular information correlated with high-grade CIN and SCC

  • correlation
  • high-grade cervical intraepithelial neplasia
  • human nonmetastatic clone 23
  • human telomerase reverse transcriptase
  • squamous cell carcinoma

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Footnotes

  • 1 The first and second authors contributed equally to the work.

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