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Human ovarian cancer ascites fluid contains a mixture of incompletely degraded soluble products of fibrin that collectively possess an antiangiogenic property
  1. N. Jandu*,
  2. M. Richardson*,,
  3. G. Singh*,,
  4. H. Hirte*, and
  5. M. W.C Hatton*
  1. *Department of Pathology and Molecular Medicine, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada
  2. Juravinski Cancer Centre, Hamilton, Ontario, Canada
  1. Address correspondence and reprint requests to: Mark W.C. Hatton, PhD, Department of Pathology and Molecular Medicine, (Room HSC-4N67), McMaster University Health Sciences Centre, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5. Email: hattonm{at}


Ovarian cancer ascites fluid (OCAF) displayed an antiangiogenic property in a chick chorioallantoic membrane (CAM) assay. This property was attributed in part to angiostatin although angiostatin-free OCAF retained a net antiangiogenic property. Recently, immunopurified fibrin(ogen) degradation products (FDPs) from malignant effusions of VX2 tumor–burdened rabbits exhibited antiangiogenic activity on the CAM. We questioned whether the FDPs of OCAF were also antiangiogenic. FDPs were immunopurified from individual OCAF samples, characterized by sodium dodecyl sulfate–polyacrylamide gel electrophoresis /western blots, enzyme-linked immunosorbent assays, and CAM assays. FDPs of OCAFs consisted of soluble high molecular weight (MW) fragments (>200 kd; ∼40% of total FDPs), D-dimer (∼180 kd; ∼37%), fragment D (∼90 kd; ∼15%), and fragment E (∼50 kd; ∼8%); intact fibrinogen was absent. When applied to CAM surfaces (0.5–1.6 mg/10 mL), purified FDPs significantly reduced the area of chorionic capillaries from 90% (in controls) to 47% over a 48-h period; from CAM sections, capillary density was reduced from 60% (controls) to 26%. FDPs prepared from fibrinogen displayed a similar antiangiogenic effect. Further digestion of OCAF FDPs by human plasmin caused degradation of high MW fragments, releasing additional D-dimer, fragment D, and fragment E. Of the fibrinogen-related components, OCAF contained only soluble FDPs (including incompletely digested fibrin fragments). Collectively, these FDPs contributed to the net antiangiogenic property of ascites fluid.

  • antiangiogenesis
  • ascites fluid
  • chorioallantoic membrane
  • fibrin degradation products
  • ovarian cancer

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