Article Text

Download PDFPDF

X chromosomal and autosomal loss of heterozygosity and microsatellite instability in human cervical carcinoma
  1. J. Edelmann*,
  2. K. Richter*,
  3. C. HÄNEL,
  4. S. Hering and
  5. L. C. Horn
  1. * Institute of Legal Medicine, University of Leipzig, Leipzig, Germany
  2. Institute of Pathology, University of Leipzig, Leipzig, Germany
  3. Institute of Legal Medicine, Technical University of Dresden, Dresden, Germany
  1. Address correspondence and reprint requests to: Dr Jeanett Edelmann, PhD, Institute of Legal Medicine, University of Leipzig, Johannisallee 28, D–04103 Leipzig, Germany. Email: jeanett.edelmann{at}medizin.uni-leipzig.de

Abstract

The study analyzes tumor material and normal tissue from 27 patients with pure squamous cell carcinoma of the uterine cervix for loss of heterozygosity (LOH) and microsatellite instability (MSI) on 14 autosomal and 11 X chromosomal loci. Overall, 4–40% of the informative cases showed LOH at autosomal regions with the highest frequency at 3p (21–40%) and a marked frequency at 2q35-q37.1 (12.5%) and 17p13.3 (10%), representing regions with putative tumor suppressor gene (TSG) function. The frequency of X chromosomal LOH ranged from 4% to 20%, with a maximum at Xq28 (20%) and Xq11.2-q12 (17%), again indicating alterations in TSG. A 12% LOH was seen at Xq21.33-q22.3, a region encoding a protein with a regulatory function in the cell cycle via cyclin-dependent kinases. MSI was detected in autosomal regions in up to 7% in regions linked to the X chromosome in up to 11%, probably indicating alterations of mismatch repair mechanisms. Our results and those obtained from the literature suggest that autosomal LOH and MSI in carcinomas of the cervix uteri are predominantly found at regions with putative TSG function. Beside TSG alterations, X chromosomal LOH is probably more strongly connected to disturbances in cell cycle regulation.

  • cervical carcinoma
  • genomic instability
  • loss of heterozygosity
  • X chromosome

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.