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Analysis of differential protein expression by cisplatin treatment in cervical carcinoma cells
  1. E. K. Yim,
  2. K. H. Lee,
  3. C. J. Kim and
  4. J. S. Park
  1. Department of Obstetrics and Gynecology, Catholic University Medical College, Seoul, Republic of Korea
  1. Address correspondence and reprint requests to: Chan-Joo Kim, MD, and Jong-Sup Park, MD, Department of Obstetrics and Gynecology, Catholic University Medical College, 505 Banpo-dong, Seocho-gu, 137-040 Seoul, Republic of Korea. Email: chanjoo{at}; jspark{at}


Cisplatin (cis-diaminedichloroplatinum), a DNA-damaging agent, which readily induces apoptosis in vitro, is one of the widely used anticancer drug in the treatment of human malignancies. Cisplatin has played an important role in cervical cancer management for effective chemotherapeutic regimen, but the underlying mechanisms inducing cell death at protein level are unknown. Using proteome analysis, an investigation aimed at a better understanding of the antiproliferative mechanisms by cisplatin was carried out in HeLa cervical carcinoma cells. In total, 21 protein spots were found to be differentially expressed following cisplatin treatment, of which 12 were upregulated (eg, regulator of G-protein signaling, TRAF:TNF (tumor necrosis factor) receptor-associated factor-interacting protein [I-TRAF], and cyclin-dependent kinase inhibitor p27 [p27kip1]) and 9 were downregulated (eg, myc proto-oncoprotein [c-myc] and proliferating cell nuclear antigen). Interestingly, we found the upregulation of proliferating cell nuclear antigen, which used molecular marker in cervical cancer screening. On the basis of proteomic data, we showed that cisplatin induced TRAF2-mediated NF-κB downregulation. In addition, our study demonstrated that cisplatin induced membrane death receptor–mediated and mitochondria-mediated apoptosis pathway. Our findings may offer new insights into the antiproliferative mechanism by cisplatin and its mode of action in cervical carcinoma cells.

  • apoptosis
  • cervical carcinoma cells
  • cisplatin
  • proteome analysis

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