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Arsenic trioxide–loaded, microemulsion-enhanced cytotoxicity on MDAH 2774 ovarian carcinoma cell line
  1. M. C. Terek*,
  2. B. Karabulut,
  3. N. Selvi,
  4. L. Akman*,
  5. Y. Karasulu§,
  6. I. Ozguney§,
  7. A. U. Sanli,
  8. R. Uslu and
  9. A. Ozsaran*
  1. *Department of Obstetrics and Gynecology, Ege University School of Medicine, Bornova, Izmir, Turkey
  2. Department of Internal Medicine Division of Medical Oncology, Ege University School of Medicine, Bornova, Izmir, Turkey
  3. Departments of Medical Biology, Ege University School of Medicine, Bornova, Izmir, Turkey
  4. §Departments of Pharmaceutical Technology, Ege University School of Medicine, Bornova, Izmir, Turkey
  1. Address correspondence and reprint requests to: Dr Mustafa Cosan Terek, Assistant Professor, Department of Obstetrics and Gynecology, Ege University Faculty of Medicine, Bornova, Izmir 35100, Turkey. Email: mustafa.cosan.terek{at}ege.edu.tr

Abstract

The antiproliferative effect of As2O3-loaded microemulsion (As2O3-M) on human MDAH 2774 ovarian cancer cells was compared with a regular solution of the As2O3. We used MDAH 2774 as model cell lines for ovarian cancer. The (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide) (XTT) and trypane blue dye exclusion tests were used to evaluate cytotoxicity. Apoptotic effect of solutions was evaluated using cell death detection kit. Standard microemulsion formulation used in this experiment contains 5 × 10−6 M As2O3. It was clearly demonstrated that As2O3-M had a significant cytotoxic effect on MDAH 2774 cell line, and the cytotoxic effect of As2O3-M was significantly higher than that of regular As2O3 solutions. Even approximately 6000 times diluted microemulsion formulation loaded with 5 × 10−6 M As2O3 showed a cytotoxic effect. As a result, this diluted concentration (approximately 8 × 10−10 M) was found to be approximately 6000 times more effective than regular As2O3 solutions (5 × 10−6 M). Moreover, this diluted concentration resulted in 1.5-fold enhancement of apoptosis. According to the in vitro cytotoxicity studies, we concluded that by incorporating As2O3 into the microemulsion (As2O3-M), which is a new drug carrier system, it is possible to increase antiproliferative effect of regular As2O3 on MDAH 2774 cells. Translating these results to in vivo conditions would open new windows in the treatment of ovarian cancer.

  • As2O3-loaded microemulsion
  • cytotoxicity
  • ovarian cancer

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