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Arsenic trioxide induces apoptosis in cisplatin-sensitive and -resistant ovarian cancer cell lines
  1. B. KONG*,
  2. S. HUANG*,
  3. W. WANG*,
  4. D. MA,
  5. X. QU,
  6. J. JIANG*,
  7. X. YANG*,
  8. Y. ZHANG*,
  9. B. WANG*,
  10. B. CUI* and
  11. Q. YANG§
  1. *Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Ji'nan, People's Republic of China
  2. †Department of Haematology, Qilu Hospital, Shandong University, Ji'nan, People's Republic of China
  3. ‡Department of Basic Medicine, Qilu Hospital, Shandong University, Ji'nan, People's Republic of China
  4. §The Cancer Institute of New Jersey, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey
  1. Address correspondence and reprint requests to: Beihua Kong, MD, PhD, Chairman and Director, Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Ji'nan 250012, Shandong Province, People's Republic of China. Email: kongbeihua{at}yahoo.com.cn

Abstract

Arsenic trioxide (As2O3), has been used for centuries in traditional Chinese medicine; it has considerable efficacy in the treatment of relapsed acute promyelocytic leukemia, inducing partial differentiation and promoting apoptosis of malignant promyelocytes. Although a number of studies have demonstrated that As2O3 has potent activity against cell growth in a series of leukemia cell lines, little information is available regarding this compound's effect on cell growth in solid tumor cell lines. In this study, we investigated the effects of As2O3in vitro on ovarian cancer cell lines sensitive (3AO) and resistant (3AO/CDDP) to cisplatin. The 3-(4,5-dimethy-thiazoyl-2-yl)-2,5-diphenyl-tetrazolium bromide assay was used to evaluate cytotoxicity. Flow cytometric analysis was used to determine the apoptosis, cell cycle distribution. We clearly demonstrated that As2O3 induced cell apoptosis and inhibition of cell growth in both the cell lines. Furthermore, we identified that As2O3-induced apoptosis involved Fas pathway. As2O3 is an active agent against ovarian cancer cells and could be effective in the clinical treatment of ovarian cancer.

  • apoptosis
  • As2O3
  • cell cycle
  • Fas
  • ovarian cancer

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