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Ultrastructure alterations in adriamycin-resistant and cisplatin-resistant human ovarian cancer cell lines exposed to nonlethal ultrasound
  1. T. Yu*,
  2. X. Huang,
  3. S. Jiang,
  4. K. Hu*,
  5. B. Kong and
  6. Z. Wang*
  1. * Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing, China
  2. Department of Ultrasonography, 1st Affiliated Hospital, Chongqing Medical University, Chongqing, China
  3. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China
  1. Address correspondence and reprint requests to: Tinghe Yu, Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China. Email: yutinghe{at}


Human ovarian cancer cell lines, SKOV3 and its adriamycin-resistant substrain SKOV3/ADR and COC1 and its cisplatin-resistant substrain COC1/DDP, were exposed to nonlethal ultrasound. Ultrastructures in sham-insonated and insonated cells were inspected by transmission electron microscopy, and cytochrome C in cytosol was determined by enzyme-linked immunosorbent assay. Ultrasound exposure led to no significant changes in SKOV3/ADR cells, but tumid mitochondria occurred in SKOV3 cells. Mitochondria changes were also detected in some exposed COC1 and COC1/DDP cells. Apoptotic bodies could be detected in either control or insonated COC1/DDP cells. A few exposed COC1/DDP cells became reticular. Cytochrome C in cytosol in exposed SKOV3/ADR cells was increased but that in exposed COC1/DDP cells was decreased. These findings revealed that the bioeffect of ultrasound on chemosensitive cells was not identical to that of chemoresistant ones, and ultrasound was a potential approach for treatment of drug-resistant ovarian cancers.

  • chemoresistance
  • cytochrome C
  • ovarian cancer
  • ultrasonic therapy
  • ultrastructure

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