Article Text

Download PDFPDF
Gain of chromosome 3q is an early and consistent genetic aberration in carcinomas of the vulva
  1. P. Stoltzfus*,
  2. K. Heselmeyer-Haddad,
  3. J. Castro*,
  4. N. White,
  5. C. Silfverswärd*,
  6. K. Sjövall,
  7. N. Einhorn*,
  8. K. Tryggvason§,
  9. G. Auer*,
  10. T. Ried and
  11. B. Nordström*
  1. * Department of Oncology and Pathology, Stockholm, Sweden
  2. Center for Cancer Research, NCI, Bethesda, MD, Stockholm, Sweden
  3. Department of Obstetrics and Gynecology, Karolinska Hospital, Stockholm, Sweden
  4. § Karolinska Insitutet, Stockholm, Sweden
  1. Address correspondence and reprint requests to: Britta Nordström, MD, PhD, Radiumhemmet, Karolinska Hospital, 171 76 Stockholm, Sweden. Email: brita.nordstrom{at}


The aim was to determine whether specific gains of chromosome 3q and laminin-5γ2-chain expression can improve early detection of invasive capacity in precancerous and squamous cell carcinoma of the vulva (VSCC). Six VSCC and three precancerous lesions were studied. Multicolor fluorescence in situ hybridization (FISH) probe sets were applied to nuclei suspensions prepared from archival material using the Hedley method. The probe panel consists of the centromers of chromosome 7, chromosome 3, and the TERC gene residing on the long arm of chromosome 3. Laminin-5γ2-chain immunohistochemical analysis was performed on corresponding specimens and was expressed only in the VSCC. The genome-specific FISH analysis revealed 3q amplification in 43% of the nuclei analyzed for the VSCC and 22% of the nuclei for the precancerous lesions. Low-level 3q amplifications were found in precancerous lesions with an average fold increase of 1.15 for 3q. The invasive lesions showed higher average fold increases for 3q, averaging 1.32. Laminin-5γ2-chain protein was expressed only in VSCC, whereas 3q gains were observed both in precancerous lesions and in VSCC, indicating that gain of chromosome 3q is an early and consistent event during carcinogenesis of VSCC.

  • chromosome 3q
  • laminin-5γ2 chain
  • VIN
  • vulvar carcinoma

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.