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Effect of daidzein on cell growth, cell cycle, and telomerase activity of human cervical cancer in vitro
  1. J. M. Guo*,
  2. G. Z. Kang,
  3. B. X. Xiao*,
  4. D. H. Liu and
  5. S. Zhang
  1. * School of Medicine, Ningbo University, Ningbo, China
  2. Department of Biochemistry, Gannan Medical College, Ganzhou, China
  3. Ningbo No. 2 Hospital, Ningbo, China
  1. Address correspondence and reprint requests to: Jun Ming Guo, MD, PhD, School of Medicine, Ningbo University, Ningbo 315211, China. Email: junmingguo{at}


Phytoestrogens are some plant compounds exhibiting estrogen-like activities. However, some studies have shown that they also affect the growth of some nonhormone-dependent diseases. In this study, daidzein – one of the most common phytoestrogens – was used to investigate its effects on human cervical cancer cells HeLa in vitro. First, the cell growth was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Then, the distributions of cell cycle and apoptosis were analyzed with the help of flow cytometry. Finally, the telomerase activity was detected by using real-time quantitative reverse transcription-polymerase chain reaction. The results showed that at the concentrations from 6.25 to 100 μmol/l, daidzein inhibited the growth of HeLa cells. Flow cytometric analysis showed that cancer cells were arrested at G0 / G1 or G2 / M phase with daidzein. The inductive effects of apoptosis were more obviously observed in low-concentration groups. After HeLa cells were treated with daidzein, the expression of human telomerase catalytic subunit mRNA decreased. These meant that daidzein affected human nonhormone-dependent cervical cancer cells in several ways, including cell growth, cell cycle, and telomerase activity in vitro.

  • cell cycle
  • cell growth
  • cervical tumor
  • daidzein
  • telomerase

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