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Scutellaria barbata D. Don induces c-fos gene expression in human uterine leiomyomal cells by activating β2‐adrenergic receptors
  1. T.-K. LEE*,
  2. H.-L. CHO*,
  3. D.-I. KIM*,
  4. Y.-C. LEE and
  5. C.-H. KIM*
  1. *Department of Biochemistry, Molecular Biology and Gynecology, Dongguk University College of Oriental Medicine, and National Research Laboratory for Glycobiology, Korean Ministry of Science and Technology, Kyungju City, Kyungbuk, Korea
  2. Facuty of Biotechnology, Dong-A University, Saha-Gu, Busan, Korea
  1. Address correspondence and reprint requests to: Dr Cheorl-Ho Kim, Department of Biochemistry and Molecular Biology, Dongguk University COM, Sukjang-Dong 707, Kyungju City, Kyungbuk 780-714, Korea. Email: chkimbio{at}


Scutellaria barbata D. Don (Lamiaceae; SB) inhibited the growth of uterine leiomyomal (LM) cells with unknown actions. The expression patterns of β-adrenergic receptors (β-ARs) in human uterine LM cells and functional coupling to gene expression have also been investigated. Northern blot analysis showed that β-AR subtypes are expressed at different levels in the uterine LM cells and myometrial smooth muscle cells (SMCs). β1-AR expression was to be found approximately at the same level in the two cell types. β2-ARs were expressed at higher levels in uterine LM cells than that in myometrial SMCs. β3-AR expression was not found in both the cells. c-fos gene expression was induced by SB in uterine LM cells via increases in adenosine-3′,5′, cyclic monophosphate (cAMP), which in turn activated the cAMP/protein kinase A (PKA) pathway. The PKA inhibitor, H89, inhibited c-fos gene expression induced by SB. It seems that the mechanism of proto-oncogenes c-fos different leiomyoma from other myometrial cancer. Further studies are necessary to elucidate whether c-fos induction by SB in uterine LM cells influences a regression of leiomyoma or induces other differentiation.

  • adenylate cyclase
  • β-adrenergic receptor
  • β1-AR
  • β2-AR
  • β3-AR
  • cAMP
  • c-fos
  • myometrial smooth muscle cells
  • PKA
  • proto-oncogenes
  • Scutellaria barbata D. Don (Lamiaceae)
  • uterine leiomyomal cells

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