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ORIGINAL ARTICLES
Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells
  1. L. Lelievre,
  2. P. Paterlini-Brechot,
  3. S. Camatte,
  4. E. Tartour,
  5. M. Aggerbeck,
  6. F. Vilde and
  7. F. Lecuru
  1. * Service de Chirurgie Gynécologique et Oncologique, Hôpital Européen Georges Pompidou, Paris, France
  2. † Unité Inserm U370, Faculté de Médecine Necker-Enfants Malades, Paris, France
  3. ‡ Unité d'Immunologie Biologique, Hôpital Européen Georges Pompidou, Paris, France
  4. § Unité Inserm U190, Université René Descartes, Faculté de Médecine;, Paris, France
  5. Service d'Anatomie Pathologique, Hôpital Européen Georges Pompidou, Paris, France
  1. Address correspondence and reprint requests to: Lelievre Loïc, Service de Chirurgie Gynécologique et Oncologique, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75015 Paris, France. Email: lelievre.loic{at}wanadoo.fr

Abstract

Aim To assess the effect of laparoscopy on circulating tumor cell (CTC) detection in case of carcinosis.

Material and methods We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO2 laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.

Results Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one postexperiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).

Conclusion This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.

  • carcinosis
  • circulating tumor cells
  • ISET
  • laparoscopy
  • ovarian cancer

https://doi.org/10.1136/ijgc-00009577-200403000-00008

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