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Uterine response to estradiol under action of chorionic gonadotropin in mice
  1. A. G. Gunin,
  2. V. U. Emelianov,
  3. I. U. Mironkin,
  4. M. P. Morozov and
  5. V. A. Ivanov
  1. Department of Histology, Medical School, Chuvash State University, Cheboksary, Russia
  1. Address correspondence and reprint requests to: Dr. Andrei G. Gunin, P.O. Box 86, Cheboksary, 428034, Russia. Email: drgunin{at}mail.ru or drgunin{at}yahoo.com

Abstract

This work examined the effect of chorionic gonadotropin on proliferative and morphogenetic reactions in the uterus under short- and long-term estrogen treatments. Ovariectomized mice received a single injection with estradiol dipropionate (2 μg per 100 g; subcutaneously, sc) or vehicle and injections with human chorionic gonadotropin (10 IU per 100 g; sc) or vehicle twice a day for 2 days. Other groups of animals received injections with estradiol once a week or vehicle and injections with chorionic gonadotropin or vehicle once a day for 30 days. The uteri were removed 48 h after the last estradiol or vehicle injection. In animals treated with estradiol and chorionic gonadotropin for a month, the incidence of atypical endometrial hyperplasia was significantly higher. In animals treated with estradiol and chorionic gonadotropin for 2 days or for a month, uterine mass was slightly increased, the number of mitotic cells and BrdU-labeled cells was greater in luminal epithelium, glandular epithelium, stromal and myometrial cells, whereas the expression of estrogen receptors-α was lower in all uterine compartments, than in control. In mice who received estradiol and chorionic gonadotropin for 2 days, levels of β-catenin and glycogen synthase kinase-3β in luminal and glandular epithelia were lower. In animals treated with estradiol and chorionic gonadotropin for a month, the level of β-catenin was slightly higher, and the expression of glycogen synthase kinase-3β was lower in luminal and glandular epithelia. Thus, chorionic gonadotropin exerts estradiol-induced proliferative and morphogenetic changes in the uterus. This action of chorionic gonadotropin is associated with decreased expression of estrogen receptors-α and with changes in expression of β-catenin and glycogen synthase kinase-3β in the uterus.

  • β-catenin
  • chorionic gonadotropin
  • endometrial hyperplasia
  • estrogen receptors
  • estrogens
  • glycogen synthase kinase-3β
  • proliferation
  • uterus

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