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Expression of interleukin-8 in human metastatic endometrial carcinoma cells and its regulation by inflammatory cytokines
  1. K. K. Berry1,
  2. M. L. Varney2,
  3. B. J. Dave2,
  4. C. D. Bucana1,
  5. I. J. Fidler1 and
  6. R. K. Singh2
  1. 1Department of Cancer Biology, University of Texas, M. D. Anderson Cancer Center, Houston, Texas and
  2. 2Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska
  1. Address correspondence and reprint requests to: R.K. Singh, PhD, Department of Pathology and Microbiology, University of Nebraska Medical Center, 987660 Nebraska Medical Center, Omaha, NE 68198-7660. Email: rsingh{at}


In the present study, we analyzed the expression of a multifunctional cytokine, interleukin-8 (IL-8), in metastatic endometrial carcinoma cells. Our data demonstrate that human serous papillary endometrial adenocarcinoma (SPEC) and human endometrial adenocarcinoma (HEC) cells expressed steady-state IL-8-specific mRNA transcript and secreted IL-8 protein. The levels of IL-8 mRNA in SPEC-2 cells established from stage IV serous papillary adenocarcinoma were three-fold higher as compared to endometrial adenocarcinoma cells, HEC-1 A, established from stage IA endometrial cancer. Further, we observed higher levels of IL-8 mRNA and protein expression in the metastatic variants of SPEC-2 and HEC-1A cells as compared to the parent cell lines, demonstrating that IL-8 expression was associated with metastatic potential. Further, the treatment of endometrial carcinoma cells with inflammatory cytokines, IL-1β and tumor necrosis factor-α (TNF-α), demonstrated that IL-1β and TNF-α induced IL-8 expression in endometrial cancer cells. IL-1β was a more potent inducer of IL-8 expression than TNF-α in our studies. These data demonstrate that constitutive and induced IL-8 expression in endometrial carcinoma cells might be an important regulatory mechanism of tumor growth and metastasis.

  • cytokine
  • interleukin-1
  • interleukin-8
  • metastasis

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