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Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma
  1. G. Lapertosa1,2,
  2. P. Baracchini1,
  3. F. Cabiddu1,
  4. E. Fulcheri1,
  5. F. PedullÀ1,* and
  6. N. Ragni1,*
  1. 1 Institutes of Pathological Anatomy and Histology, University of Genoa, Italy
  2. 2 Professor G. Lapertosa, Institute of Pathological Anatomy and Histology, University of Genoa, via De Toni 14, 16132 Genoa, Italy.
  3. * Gynaecology and Obstetrics, University of Genoa, Italy


Out of a total of 1050 lymph nodes from patients with clinical squamous carcinoma, 58 lymph node metastases related to 18 patients were detected with H&E staining, while 61 lymph node metastases related to 19 patients were observed by immunohistochemical methods. However, by re-staining the original H&E sections with an anti-keratin antibody, the same 58 lymph node metastases were seen. Therefore, the three metastases emerged only in deeper sections which had been used for the immunohistochemical examination. It is suggested that the detection of micrometastases in lymph nodes may be improved by examining several sections at different levels rather than by immunohistochemical methods, even though these facilitate the observation of small groups of neoplastic cells.

  • immunohistochemistry
  • keratin antibodies
  • lymph node micrometastases
  • squamous cell carcinoma
  • uterine cervix.

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